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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
齐源
- 库存：
999
- 靶点：
CD43 Activation
- 级别：
CD43 Activation-Associated Glycoform Antibody
- 目录编号：
CD43 Activation-Associated Glycoform Antibody
- 克隆性：
单克隆
- 抗原来源：
大鼠
- 保质期：
CD43 Activation-Associated Glycoform Antibody
- 抗体英文名：
Cyanine7 anti-mouse CD43 Activation-Associated Glycoform Antibody-APC
- 抗体名：
CD43 Activation-Associated Glycoform Antibody
- 标记物：
APC
- 宿主：
大鼠
- 适应物种：
小鼠
- 免疫原：
CD43 Activation-Associated Glycoform Antibody
- 亚型：
CD43 Activation-Associated Glycoform Antibody
- 形态：
CD43 Activation-Associated Glycoform Antibody
- 应用范围：
FC
- 保存条件：
2-8℃
- 浓度：
0.2 mg/ml
- 规格：
25 µg/100 µg
| 规格： | 25 µg | 产品价格： | ￥2400.0 |
|---|---|---|---|
| 规格： | 100 µg | 产品价格： | ￥5500.0 |
Description ：CD43, also known as Ly-48, Leukosialin, Sialophorin, Leukocyte Sialoglycoprotein, and gp115,is a large single chain of type I transmembrane glycoprotein with abundant O-glycosylation andsialylation sites. Due to variable glycosylation and sialylation, two isoforms of CD43 have beenidentified. The 115 kD glycoform of CD43 is expressed on most hematopietic cells including Tlymphocytes, NK cells, monocytes, granulocytes, platelets, and CD5 B cells. It is not presenton resting B cells and erythrocytes. While the 130 kD glycoform is thought to be activationassociated form primarily expressed on myeloid cells, pre-B cells, and activated T cells. It hasbeen reported that CD43 binds to CD54 and Siglec-1. CD43 plays dual roles in cell adhesionand anti-adhesion, as well as costimulation of T cell activation and survival, and induction ofapoptosis of T cells and hematopoietic progenitors. The 1B11 antibody reacts with theactivation-associated glycoform of CD43. The epitope recognized by 1B11 is also shared withdesialylated CD45RB. This antibody is useful for differentiation of effector CD8 T cells andmemory T cells.
Verified Reactivity ：Mouse
Antibody Type ：Monoclonal
Host Species ：Rat
Immunogen ：Mouse WEHI 274.3 myeloid tumor cells
Formulation ：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation ：The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 underoptimal conditions.
Concentration ：0.2 mg/ml
Storage & Handling ：The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application ：FC - Quality tested
Recommended Usage ：Each lot of this antibody is quality control tested by immunofluorescent staining with flowcytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µgper million cells in 100 µl volume. It is recommended that the reagent be titrated for optimalperformance for each application.
Excitation Laser ：Red Laser (633 nm)
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文献和实验1. Jones AT, et al. 1994. J. Immunol. 153:3426. (IP, WB)
2. Carlow DA, et al. 1999. J. Immunol. 163:1441. (IP, WB)
3. Onami TM, et al. 2002. J. Immunol. 168:6022.
4. van der Most RG, et al. 2003. Intl. Immunol. 15:119.
5. Chu VT, et al. 2007. J. Immunol. 179:5947.
6. Lang A, et al. 2008. J. Immunol. 180:4848. PubMed
7. Gibbert K, et al. 2010. J. Immunol. 185:6179. PubMed
Product Citations：
1. Verma S, et al. 2016. J Virol. 90: 650 - 658. PubMed
2. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
3. Shikatani EA et al. 2019. Cell Rep. 27(8):2304-2312 . PubMed
4. Shan M et al. 2018. Immunity. 49(4):709-724 . PubMed
5. Oladiran O, et al. 2021. J Neuroinflammation. 77:18. PubMed
6. Isvoranu G, et al. 2019. Oncol Lett. 17:4197. PubMed
流式抗体的选择和配色原则由于在流式细胞实验过程中，荧光抗体对单细胞悬液的标记效果直接影响实验的数据质量。因此，需要考虑各种影响流式抗体品质及检测效果的因素，例如抗体特异性、荧光素信号强弱、荧光素标记方式、同型对照等。本文介绍如何选择合适的流式抗体： 1、需要满足抗体选择的最基本条件：目标蛋白特异性，反应种属以及应用实验等。 靶标特异性：确定标记物名称，确定是细胞表面表达，还是细胞内表达。细胞内表达需要进行透膜。 物种来源：确定待测样本的种属来源。例如，抗小鼠的抗体，不能用于人的样本的检测
有了质量好的抗原，还必须选择适当的免疫途径，才能产生质量好（特异性强和效价高）的抗体。 （一）用于免疫的动物 作免疫用的动物有哺乳类和禽类，主要为羊、马、家兔、猴、猪、豚鼠、鸡等，实验室常用者为家兔、山羊和豚鼠等。动物种类的选择主要根据抗原的生物学特性和所要获得抗血清数量，如一般制备抗r-免疫球蛋白抗血清，多用家兔和山羊，因动物反应良好，而且能够提供足够数量的血清，用于免疫的动物应适龄
面，抗体的结合可以增强免疫细胞对抗原的识别和吞噬能力，进而增加免疫应答。另一方面，抗体的结合可以通过与免疫细胞表面的调节受体结合，抑制或增强免疫应答反应，帮助免疫系统在抗感染，抗肿瘤等过程中取得平衡的应答反应。 4、 抗原抗体复合物通过经典途径激活补体系统 补体系统是一种能够引起炎症和细胞溶解的蛋白质系统，IgG1、IgG2、IgG3和IgM类抗体与抗原结合形成免疫复合物激活补体系统，从而发挥补体介导的杀菌、溶菌作用。清除细菌、病毒等病原体。这主要在抗细菌和抗寄生虫感染中起作用。 5、介导细胞
