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文献和实验Ribonuclease A (RNase A), DNase-free
pancreas.Molecular Weight13.7 kDa monomer.ApplicationsPlasmid and genomic DNA preparation (3, 4), ..Removal of RNA from recombinant protein preparations.Ribonuclease protection assays (3).Mapping single-base mutations in DNA or RNA (5, 6).Quality Control
Primary MicroRNA Processing Assay Reconstituted Using Recombinant Drosha and DGCR8
protein DiGeorge critical region 8 (DGCR8). DGCR8 has been shown to tightly bind an Fe(III) heme cofactor, which activates its pri-miRNA processing activity. Here we describe how to reconstitute pri-miRNA processing using recombinant human Drosha and DGCR8
Preparation of Recombinant Protein Spotted Arrays for Proteome‐Wide Identification of Kinase Targets
) GST‐3C (prepared in‐house) or PreScission 3C protease (GE Healthcare Life Sciences, cat. no. 27‐0843‐01) Histone H1 (Sigma
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