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- Biomatik
- CAF50528
- 2025年07月16日
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- 库存：
10
- 规格：
100ug
Antibody TypePrimary AntibodyRabbitClonalitypolyclonalApplicationELISA, WBGene IDUnitProtStorageStore at 2°C to 8°C for frequent use, -20°C for 12 months
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文献和实验相关实验
) Remove supernatant (lysate) to another tube.
suspension into a centrifuge tube.Gently rock the suspension on either a rocker or an orbital shaker in the cold room for 15 minutes to lyse cells. 4.Centrifuge the lysate at 14,000 x g in a precooled centrifuge for 15 minutes.Immediately transfer
Summary How to make a lysate from a plaque preparation. We also use this protocol for preparation of a quick stock from previously made lysate prep. Protocol Add 5mL fresh overnight (BL21 if wild-type T7) into 15 mL of T
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DH5a-lysate antibody
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