β-hydroxybutyryl-HIST1H3A (K9)

secondary antibody review -- data from 99 publications

cytometry   used as a control to detect cell responses targeted antigen   7       Alexa Fluor 488         7       Cy3         8 goat IgG   Alexa Fluor 488   1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey

Competing Chromosomal Proteins from Drosophila Polytene Chromosomes Using Modified Histone Peptides

to chromatin, by its histone tail interaction or by other interactions, then you would not expect to see loss of binding upon peptide competition. A good positive control for the assay is to use H3K9 dimethylated peptide to compete HP1 from the pericentric

Chromatin immunoprecipitation: optimization, quantitative analysis and data normalization

. Figure 1. Outline of the ChIP-QPCR procedure. This outline represents the ChIP procedure as described in the text. IP, ChIP sample; NoAb, No-antibody control. In current literature, conventional PCR is mostly used to analyze plant ChIP