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- 齐源
- 127643
- 国产/进口
- 2025年07月12日
- FC
- 大鼠
- 小鼠
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
齐源
- 库存：
999
- 靶点：
Ly-6G
- 级别：
Ly-6G Antibody
- 目录编号：
Ly-6G Antibody
- 克隆性：
单克隆
- 抗原来源：
大鼠
- 保质期：
Ly-6G Antibody
- 抗体英文名：
Brilliant Violet 711™ anti-mouse Ly-6G Antibody
- 抗体名：
Ly-6G Antibody
- 标记物：
Ly-6G Antibody
- 宿主：
大鼠
- 适应物种：
小鼠
- 免疫原：
Ly-6G Antibody
- 亚型：
Ly-6G Antibody
- 形态：
Ly-6G Antibody
- 应用范围：
FC
- 保存条件：
2-8℃
- 浓度：
0.2 mg/ml
- 规格：
50 µg
Description ：Lymphocyte antigen 6 complex, locus G (Ly-6G), a 21-25 kD GPI-anchored protein, isexpressed on the majority of myeloid cells in bone marrow and peripheral granulocytes.
Verified Reactivity ：Mouse
Antibody Type ：Monoclonal
Host Species ：Rat
Immunogen ：Ly-6G transfected EL-4J cell line.
Formulation ：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation ：The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 711™under optimal conditions.
Concentration ：0.2 mg/ml
Storage & Handling ：The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application ：FC - Quality tested
Recommended Usage ：Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cellsin 100 µl volume. It is recommended that the reagent be titrated for optimal performance for eachapplication.
Brilliant Violet 711™ excites at 405 nm and emits at 711 nm. The bandpass filter 710/50 nm isrecommended for detection, although filter optimization may be required depending on otherfluorophores used. Be sure to verify that your cytometer configuration and software setupare appropriate for detecting this channel. Refer to your instrument manual or manufacturerfor support. Brilliant Violet 711™ is a trademark of Sirigen Group Ltd.
Excitation Laser ：Violet Laser (405 nm)
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文献和实验1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. (FC)
2. Daley JM, et al. 2008. J. Leukocyte Biol. 83:1. (FC)
3. Dietlin TA, et al. 2007. J. Leukocyte Biol. 81:1205. (FC)
4. Daley J, et al. 2007. J. Leukocyte Biol. doi:10.1189. (Deplete) PubMed
5. Tadagavadi RK, et al. 2010. J. Immunol. 185:4904. PubMed
6. Sumagin R, et al. 2010. J. Immunol. 185:7057. PubMed
7. Guiducci C, et al. 2010. J. Exp Med. 207:2931. PubMed
8. Fujita M, et al. 2011. Cancer Res. 71:2664. PubMed
9. Van Leeuwen, et al. 2008. Arterioscler. Thromb. Vasc. Biol. 28:84. (IHC)
10. Kowanetz M, et al. 2010. P. Natl. Acad. Sci. USA 107:21248. [supplementary data] (IHC)
11. Esbona K, et al. 2016. Breast Cancer Res. 18:35. (IHC)
12. Wojtasiak M, et al. 2010. J. Gen. Virol. 91:2158. (FC, Deplete)
13. Jaeger BN, et al. 2012. J. Exp. Med. 209:565. (Deplete)
14. Wozniak KL, et al. 2012. BMC Immunol. 13:65 (FC, Deplete)
15. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
16. Ng LG, et al. 2011. J Invest. Dermatol. 131:2058. PubMed
17. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
18. McCartney-Francis, N, et al. 2014. J Leukoc. Biol. 96:917. PubMed
19. Her Z, et al. 2014. EMBO Mol. Med. 7:24. PubMed
20. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
21. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations：
1. Farsakoglu Y et al. 2019. Cell reports. 26(9):2307-2315 . PubMed
2. Wang Y, et al. 2021. Sci Rep. 1.429861111. PubMed
3. Brigas HC, et al. 2021. Cell Reports. 36(9):109574. PubMed
4. Olson GS, et al. 2021. Cell Reports. 35(9):109195. PubMed
5. Seydoux E, et al. 2021. Cell Reports. 35(5):109084. PubMed
6. Liu Z, et al. 2020. Cell. 178(6):1509-1525.e19.. PubMed
7. Clark JT, et al. 2021. eLife. 10:00. PubMed
8. Zhang X, et al. 2021. Mol Cancer Res. 19:1076. PubMed
9. Pfirschke C, et al. 2020. Cell Rep. 32:108164. PubMed
10. Voeller J, et al. 2019. J Immunother Cancer. 0.530555556. PubMed
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流式抗体的选择和配色原则由于在流式细胞实验过程中，荧光抗体对单细胞悬液的标记效果直接影响实验的数据质量。因此，需要考虑各种影响流式抗体品质及检测效果的因素，例如抗体特异性、荧光素信号强弱、荧光素标记方式、同型对照等。本文介绍如何选择合适的流式抗体： 1、需要满足抗体选择的最基本条件：目标蛋白特异性，反应种属以及应用实验等。 靶标特异性：确定标记物名称，确定是细胞表面表达，还是细胞内表达。细胞内表达需要进行透膜。 物种来源：确定待测样本的种属来源。例如，抗小鼠的抗体，不能用于人的样本的检测
8, CD14, CD19； 小鼠： CD4, CD8a, CD14, CD45R/B220, CD90.2 (Thy1.2), CD11b, Ly-6G. ë 利用Streptavidin连接的磁珠，只需选配biotin标记的所需单抗,就能分离更多种类细胞 新货聚焦： 现在，BD PMG又推出用于分离小鼠造血干细胞/祖细胞的新试剂mouse lineage panel。 其中包括5种biotin标记单抗
与细胞表面相应抗原特异性结合而使细胞被磁珠间接捕获，从而达到分离。这种磁珠使研究者可根据自己需要选择生物素标记的各种单抗去分离目的细胞，应用范围更广泛，使用更灵活。 十、不同物种磁珠分离试剂 1、人CD3，CD4，CD8，CD14，CD19； 2、小鼠CD4，CD8a，CD14，CD45R/B220，CD90.2（Thy1.2），CD11b，Ly-6G。利用Streptavidin连接的磁珠，只需选配biotin标记的所需单抗
