Description :Mucin-1 (MUC-1), cell surface associated or polymorphic epithelia mucin, is a 500-1000 kDproteoglycan expressed by activated T cells, mucosal epithelial cells, and aberrantly expressedon most breast cancers. In normal cells, CD227 is heavily glycosylated, whereas in cancerouscells, the glycosylation is incomplete and premature sialation is also observed. The protein isanchored to the a-pical surface of the epithelial cell and functions as a lubricant to keep the cellhydrated and to protect against pathogens. It can also function as a signaling molecule byforming a MUC-1/SOS1/GrB2 complex. MUC-1 can interact with cancer antigens such asHer2/neu.
Recommended Usage :Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cellsin 100 µL volume. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections,a concentration range of 1 - 10 µg/mL is suggested. It is recommended that the reagent be titratedfor optimal performance for each application.