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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
999
- 靶点:
Ganglioside GD2
- 级别:
Ganglioside GD2 Antibody
- 目录编号:
Ganglioside GD2 Antibody
- 克隆性:
单克隆
- 抗原来源:
小鼠
- 保质期:
Ganglioside GD2 Antibody
- 抗体英文名:
Cyanine7 anti-human Ganglioside GD2 Antibody-APC
- 抗体名:
Ganglioside GD2 Antibody
- 标记物:
APC
- 宿主:
小鼠
- 适应物种:
人
- 免疫原:
Ganglioside GD2 Antibody
- 亚型:
Ganglioside GD2 Antibody
- 形态:
Ganglioside GD2 Antibody
- 应用范围:
FC
- 保存条件:
2-8℃
- 浓度:
Ganglioside GD2 Antibody
- 规格:
25 tests/100 tests
| 规格: | 25 tests | 产品价格: | ¥3600.0 |
|---|---|---|---|
| 规格: | 100 tests | 产品价格: | ¥7300.0 |
Description :Ganglioside GD2 is a sialic acid-containing glycosphingolipid involved in cell attachment to theextracellular matrix. Expression of GD2 in normal tissue is restricted to cells from the centralnervous system, peripheral nerves, skin melanocytes, and mesenchymal stem cells. HoweverGD2 is highly expressed by tumors of neuro-ectodermal origin such as melanomas, gliomas,neuroblastomas, and small cell lung carcinoma. GD2 has been proposed as a marker for somecancer stem cells.
Verified Reactivity :Human
Antibody Type :Monoclonal
Host Species :Mouse
Immunogen :Neuroblastoma cell line LAN-1
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation :The antibody was purified by affinity chromatography and conjugated with APC/Cyanine7 underoptimal conditions.
Concentration :Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysis online tool.)
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application :FC - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent betitrated for optimal performance for each application.
Excitation Laser :Red Laser (633 nm)
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文献和实验1. Mujoo K, et al. 1989. Cancer Res. 49:2857. (Cyt)
2. Kowalczyk A, et al. 2009. Cancer Lett. 281:171. (Apop, Cyt)
3. Battula VL, et al. 2012. J. Clin. Invest. 122:2066. (FC)
通过免疫组化的方法分析侵袭性乳腺癌中 GD2 表达的临床和病理相关性
来自纽约癌症中心的学者,对 396 例乳腺癌组织免疫组化染色 GD2 靶点,其结果与临床病理特征、生存结果进行相关性分析,结果支持 GD2 可以作为潜在治疗靶点。 双唾液酸神经节苷脂 GD2 是一种细胞表面相关抗原,在神经外胚层来源的肿瘤上表达,可作为某些癌症类型的免疫治疗靶点。但目前关于乳腺癌和 GD2 表达相关性的研究资料较少,来自纽约癌症中心的学者们研究了 GD2 作为潜在靶向治疗中生物标志物的可能性。研究团队收集了 396 例不同组织学类型的乳腺癌组织,用抗 GD2 3F8 单克隆抗体
69、CD38 等活化指标,及 IFN-γ、IL-4、IL17A 等细胞因子,则需要设置同型对照,实现阴阳性细胞群的良好区分。 CD4 CD25 (2)在某些特殊情况下,如单核细胞被 PE/Cyanine 7、APC/Cyanine 7 等串联染料标记的抗体染色时,使用同型对照抗体是十分有必要的。单核细胞一般倾向于与 Cyanine7 等染料非特异性结合,使用同型对照抗体有助于获得理想结果。 (3)不清楚抗原表达情况的时候,一定要使用同型对照来界定阴性细胞群。 (4)胞内染色时
5.5, APC等。 常见荧光素波长分布示意图 同型对照的选择: 流式细胞实验和其他抗体相关实验有点不同的就是需要选择同型对照。同型对照,是用于消除由于抗体非特异性结合到细胞表面而产生的背景染色,相当于实验的阴性对照。同型对照选择与标记抗体同种属来源,同亚型,同荧光标记的抗体。比如:抗人的CD3的PE标记的抗体,小鼠的IgG2a。同型










