Alexa Fluor® 488 抗 ZAP70 磷酸（Ty

Alexa Fluor® 488 anti-ZAP70 Phospho (Tyr319)/Syk Phospho (Tyr352) Antibody详细如下：

Description ：ZAP70 was identified in TCR-stimulated Jurkat cells. It is an inactive cytosolic tyrosine kinasethat is recruited to a transmembrane receptor lacking intrinsic catalytic activity. ZAP70 and therelated spleen tyrosine kinase (Syk) play a critical role in T-cell development and activation.This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR)stimulation, functions in the initial step of TCR-mediated signal transduction in combination withthe Src family kinases, Lck and Fyn.

ZAP70 activation can be regulated by binding to phosphorylated ITAMs of the TCR and byphosphorylation of multiple tyrosine residues on ZAP70. Phosphorylation of Tyr-315 and Tyr319 are essential for ZAP70 positive regulation of T-lymphocyte activation whereas Tyr-292has a negative regulatory role.

Phosphorylated Y319 is a positive regulator of ZAP70, which triggers the binding of Lck andactivation of NFAT and IL-2 induction. The binding of Lck to phosphorylated Y319 promotesLck mediated phosphorylation of Y493, and facilitates activation of downstream signaling. Theimportance of Y319 phosphorylation in positive regulation of ZAP70 was confirmed in a mousemodel with Y319F ZAP70 mutant expression, which resulted in severe defects in calciummobilization and thymocyte selection.

Verified Reactivity ：Human

Antibody Type ：Monoclonal

Host Species ：Mouse

Immunogen ：Modified peptide.

Formulation ：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation ：The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488under optimal conditions.

Concentration ：Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysis online tool.)

Storage & Handling ：The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.

Application ：ICFC - Quality tested

Recommended Usage ：Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Excitation Laser ：Blue Laser (488 nm)