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- 齐源
- 306523
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- 2025年07月15日
- FC, CyTOF®
- 小鼠
- 人、猴
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
齐源
- 库存：
999
- 靶点：
CD184
- 级别：
CD184 (CXCR4) (Maxpar® Ready) Antibody
- 目录编号：
CD184 (CXCR4) (Maxpar® Ready) Antibody
- 克隆性：
单克隆
- 抗原来源：
小鼠
- 保质期：
CD184 (CXCR4) (Maxpar® Ready) Antibody
- 抗体英文名：
Purified anti-human CD184 (CXCR4) (Maxpar® Ready) Antibody
- 抗体名：
CD184 (CXCR4) (Maxpar® Ready) Antibody
- 标记物：
CD184 (CXCR4) (Maxpar® Ready) Antibody
- 宿主：
小鼠
- 适应物种：
人、猴
- 免疫原：
CD184 (CXCR4) (Maxpar® Ready) Antibody
- 亚型：
CD184 (CXCR4) (Maxpar® Ready) Antibody
- 形态：
CD184 (CXCR4) (Maxpar® Ready) Antibody
- 应用范围：
FC, CyTOF®
- 保存条件：
2-8℃
- 浓度：
1.0 mg/ml
- 规格：
100 µg
Description ：CD184, also known as fusin or CXCR4, is a 45 kD seven transmembrane G-protein-linked CXCchemokine receptor. CD184 is widely expressed on blood and tissue cells, including B and Tcells, monocytes, macrophages, dendritic cells, granulocytes, megakaryocytes/platelets,lymphoid, myeloid precursor cells, endothelial cells, epithelial cells, astrocytes, and neurons,among other tissue cells. CD184 is the receptor for CXC chemokine SDF-1, mediates bloodcell migration, and is involved in B lymphopoiesis and myelopoiesis, cardiogenesis, bloodvessel formation, and cerebellar development. CXCR4 is also a coreceptor of X4 HIV-1 and analternative receptor for some isolates of HIV-2.
Verified Reactivity ：Human, Cynomolgus, Rhesus
Reported Reactivity ：African Green, Baboon, Chimpanzee, Sooty Mangabey
Antibody Type ：Monoclonal
Host Species ：Mouse
Immunogen ：CP-MAC-infected Sup-T1 cells
Formulation ：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA
Preparation ：The antibody was purified by affinity chromatography
Concentration ：1.0 mg/ml
Storage & Handling ：The antibody solution should be stored undiluted between 2°C and 8°C.
Application ：FC - Quality tested、CyTOF® - Verified
Recommended Usage ：This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling usingMaxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue withthe protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar®Ready antibodies.
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文献和实验1. McKnight A, et al. 1997. J. Virol. 71:1692.
2. Endres MJ, et al. 1996. Cell 87:745. (Immunogen, IF)
3. Volin MV, et al. 1998. Biochem. Biophys. Res. Commun. 242:46. (ICC)
4. Berndt C, et al. 1998. P. Natl. Acad. Sci. USA 95:12556. (Block)
5. Ullrich CK, et al. 2000. Blood 96:1438. (Block)
6. Murga M, et al. 2005. Blood 105:1992. (IF)
7. Thompson BD. 2007. J. Biol. Chem. 282:9547. (FC) PubMed
8. Isnardi I, et al. 2010. Blood 115:5026. PubMed
9. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
10. Fischer T, et al. 2008. PLoS One 3:e4069.
11. Schmid BC, et al. 2004. Breast Cancer Res. Treat. 84:247. (IHC)
分别利用 CD3 与 CD28 的功能学抗体，以及 CD3/CD28 Streptamer® 激活扩增 T 细胞的实验步骤与实验结果。 CD3/CD28抗体法 1、实验步骤： 1.1 抗体包被 用无菌 PBS 将 anti-mouse CD3 抗体（克隆号：145-2C11）稀释至 5μg/ml，稀释后的抗体加入到 24 孔板中，每孔 400μl，4°C 包被过夜。（注：板子在加入抗体之前，先用无菌 PBS 润洗 2-3 遍，避免干孔）； 1.2 小鼠脾脏淋巴细胞分离（次日） 1. 将 70μm
Dynabeads® Mouse T-Activator CD3/CD28 – for physiological activation of mouse T cells
volume of Dynabeads taken from the vial (step 2). 2. Activation of Mouse T Cells 1) Start with 8 × 104 purified T cells in 100-200 µl medium in a 96-well tissue culture plate. 2) Add 2 µl Dynabeads Mouse T-Activator CD3/CD
免疫亲和层析是以 Fab 片段为基础的无痕亲和细胞分选技术（traceless affinity cell selection, Fab-TACS）。Fab-TACS 运用免疫亲和层析的原理，使用细胞表面抗原分子 CD marker 中的 Fab 片段来进行捕捉及释放目的细胞。以抗体的 Fab 片段取代一般具有高亲和力的完整抗体，实现试剂与细胞的可逆结合，直接从全血或其他血液样本中分选目的细胞。 Fab-TACS 柱子填充了包被有 Strep-Tactin® 多聚体的细胞级琼脂糖基质，偶联
