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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
999
- 靶点:
NR5A2
- 级别:
NR5A2 Antibody
- 目录编号:
NR5A2 Antibody
- 克隆性:
单克隆
- 抗原来源:
小鼠
- 保质期:
NR5A2 Antibody
- 抗体英文名:
Purified anti-NR5A2 Antibody
- 抗体名:
NR5A2 Antibody
- 标记物:
NR5A2 Antibody
- 宿主:
小鼠
- 适应物种:
人
- 免疫原:
NR5A2 Antibody
- 亚型:
NR5A2 Antibody
- 形态:
NR5A2 Antibody
- 应用范围:
WB
- 保存条件:
2-8℃
- 浓度:
0.5 mg/ml
- 规格:
100 µg
Description :The nuclear receptor subfamily 5 group A member 2, also known as NR5A2, is a 54 kD nuclearreceptor protein. NR5A2 is a member of the FTZ-F1 sub-family and is highly expressed in thepancreas and to a lesser extent in the liver. NR5A2 contains a DNA binding domain, a hormoneligand-binding domain, and two zinc finger domains. It also binds to DNA as a monomer. NR5A2is known to regulate the transcription of the liver enzyme cholesterol 7-alpha-hydrolase, whichconverts cholesterol to bile acids and also activates the hepatitis B virus enhancer II tocontribute to the liver-specific expression of this virus. NR5A2 has been suggested to regulatepancreas-specific gene expression and to play a role in embryonic development. There are atleast 3 alternatively spliced isoforms of NR5A2: isoforms 1 and 2 are present in fetal and adultliver and in liver cancer cells HepG2 as well.
Verified Reactivity :Human
Antibody Type :Monoclonal
Host Species :Mouse
Immunogen :Partial human NR5A2 recombinant protein (1-200 a.a.) expressed in E. coli.
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation :The antibody was purified by affinity chromatography.
Concentration :0.5 mg/ml
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C.
Application :WB - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by Western blotting. For Western blotting, thesuggested use of this reagent is 1.0 - 2.5 µg per ml. It is recommended that the reagent be titratedfor optimal performance for each application.
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文献和实验有了质量好的抗原,还必须选择适当的免疫途径,才能产生质量好(特异性强和效价高)的抗体。 (一)用于免疫的动物 作免疫用的动物有哺乳类和禽类,主要为羊、马、家兔、猴、猪、豚鼠、鸡等,实验室常用者为家兔、山羊和豚鼠等。动物种类的选择主要根据抗原的生物学特性和所要获得抗血清数量,如一般制备抗r-免疫球蛋白抗血清,多用家兔和山羊,因动物反应良好,而且能够提供足够数量的血清,用于免疫的动物应适龄
面,抗体的结合可以增强免疫细胞对抗原的识别和吞噬能力,进而增加免疫应答。另一方面,抗体的结合可以通过与免疫细胞表面的调节受体结合,抑制或增强免疫应答反应,帮助免疫系统在抗感染,抗肿瘤等过程中取得平衡的应答反应。 4、 抗原抗体复合物通过经典途径激活补体系统 补体系统是一种能够引起炎症和细胞溶解的蛋白质系统,IgG1、IgG2、IgG3和IgM类抗体与抗原结合形成免疫复合物激活补体系统,从而发挥补体介导的杀菌、溶菌作用。清除细菌、病毒等病原体。这主要在抗细菌和抗寄生虫感染中起作用。 5、介导细胞
,对某些传染病如甲型肝炎有较高的临床诊断价值。 抗原抗体反应 1. 可逆性 抗原与抗体结合形成抗原抗体复合物的过程是一种动态平衡,其反应式为: Ag+Ab→Ag·Ab 抗体的亲和力(affinity)是抗原抗体间的固有结合力,可以用平衡常数K表示: K=[Ag·Ab]/[Ag][Ab] Ag·Ab 的解离程度与K值有关。高亲和力抗体的抗原结合点与抗原的决定簇在空间构型上非常适合,两者结合牢固,不易解离。解离后的抗原或抗体均能保持原有的结构和活性,因此可用亲和层析法来提纯抗原或抗体。在抗










