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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
999
- 靶点:
Histone H3 Phospho
- 级别:
Histone H3 Phospho (Ser10) Antibody
- 目录编号:
Histone H3 Phospho (Ser10) Antibody
- 克隆性:
单克隆
- 抗原来源:
小鼠
- 保质期:
Histone H3 Phospho (Ser10) Antibody
- 抗体英文名:
Alexa Fluor® 647 anti-Histone H3 Phospho (Ser10) Antibody
- 抗体名:
Histone H3 Phospho (Ser10) Antibody
- 标记物:
Histone H3 Phospho (Ser10) Antibody
- 宿主:
小鼠
- 适应物种:
人
- 免疫原:
Histone H3 Phospho (Ser10) Antibody
- 亚型:
Histone H3 Phospho (Ser10) Antibody
- 形态:
Histone H3 Phospho (Ser10) Antibody
- 应用范围:
ICFC
- 保存条件:
2-8℃
- 浓度:
Histone H3 Phospho (Ser10) Antibody
- 规格:
25 tests/100 tests
| 规格: | 25 tests | 产品价格: | ¥3500.0 |
|---|---|---|---|
| 规格: | 100 tests | 产品价格: | ¥7700.0 |
Description :Histone H3 is phosphorylated at serine 10 during mitosis and is found to be involved intranscriptional activation, chromatin decondensation, and chromosome compaction during celldivision, by the action of Aurora kinase and NIMA kinases.
Verified Reactivity :Human
Antibody Type :Monoclonal
Host Species :Mouse
Immunogen :Modified synthetic peptide conjugated to KLH
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation :The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647under optimal conditions.
Concentration :Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysis online tool.)
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application :ICFC - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by intracellular immunofluorescent staining withflow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl permillion cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm
Excitation Laser :Red Laser (633 nm)
Application Notes :The histone H3 pS10 antibody recognizes phosphorylation of human H3 protein at Ser10 residueand has been shown to be useful for Western blotting.
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文献和实验1. Benedict B, et al. 2020. Developmental Cell. 52(6):683-698. PubMed
2. de Jong MRW, et al. 2020. Ther Adv Hematol. 11:2040620719898373. PubMed
保持优异的水溶性,为该波段最亮的橙色荧光染料。例如:同等的标记程度下,CF543 标记的羊抗鼠IgG抗体的亮度高于用Alexa Fluor 546 标记的2~10倍。CF 555、AlexaFluor 555、Tetramethylrhodamine(TAMRA) 等匹配Cy3滤光片的橙色荧光染料;红色(568-594nm处激发)CF 568、Alexa Fluor® 568,ATTO 565, Rhodamine Red等568nm处红色荧光染料;CF 568染料耐光性最好;高效水溶
技术原理与方法|超级色差校正物镜 PLAPON60XOSC 在脑组织四重免疫荧光分析中的应用
种类型的荧光抗体在单个标本中准确展示荧光标记分子的共定位情况。 PLAPON60XOSC 和 UPLSAPO60XO 的性能比较 2. 超级色差校正物镜的应用-脑组织的四重免疫荧光 图 1. 脑组织的四重免疫荧光。 VIAAT 染色(Alexa Fluor405,蓝色);CB1 染色(Alexa Fluor488,绿色);VGluT3 染色(Cy3,红色);DGLα染色(Alexa Fluor647,白色)。 大麻素合成酶 DGLα(白色)和大麻素受体 CB1(绿色)聚集在小鼠
,耐光,水溶性好,确保了CF 543染料与抗体的耦联物保持优异的水溶性,为该波段最亮的橙色荧光染料。例如:同等的标记程度下,CF 543标记的羊抗鼠IgG抗体的亮度高于用Alexa Fluor 546 标记的2~10倍。 CF 555、Alexa Fluor 555、Tetramethylrhodamine (TAMRA) 等匹配Cy3滤光片的橙色荧光染料。 4、红色(568-594nm处激发) CF 568、Alexa Fluor® 568, ATTO 565











