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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
齐源
- 库存：
999
- 靶点：
CD268 (BAFF-R)
- 级别：
human CD268 (BAFF-R) Antibody
- 目录编号：
human CD268 (BAFF-R) Antibody
- 克隆性：
单克隆
- 抗原来源：
小鼠
- 保质期：
human CD268 (BAFF-R) Antibody
- 抗体英文名：
Dazzle™ 594 anti-human CD268 (BAFF-R) Antibody-PE
- 抗体名：
CD268 (BAFF-R) 抗体
- 标记物：
PE
- 宿主：
小鼠
- 适应物种：
人
- 免疫原：
human CD268 (BAFF-R) Antibody
- 亚型：
human CD268 (BAFF-R) Antibody
- 形态：
human CD268 (BAFF-R) Antibody
- 应用范围：
FC
- 保存条件：
2-8℃
- 浓度：
human CD268 (BAFF-R) Antibody
- 规格：
25 tests/100 tests
| 规格： | 25 tests | 产品价格： | ￥2400.0 |
|---|---|---|---|
| 规格： | 100 tests | 产品价格： | ￥5900.0 |
Description ：B cell-activating factor receptor (BAFF-R) is a 19 kD type III membrane protein. It belongs toTNFR superfamily, also known as TNFRSF member 13C (TNFRSF13C), BAFF receptor 3(BR3), or CD268. BAFF-R is expressed on mature B cells, B cell lymphoma, and T cell subset.BAFF-R is the major receptor for BAFF/BLys (or TALL-1, THANK) which binds to TACI andBCMA as well. The interaction of BAFF with BAFF-R promotes NF-κB activation and playspredominant roles in B-cell maturation and survival as well as costimulates T cell activation andproliferation. TRAF3 is a BAFF-R intracellularly associated protein, which negatively regulatesBAFF-R-mediated NF-κB activation.
Verified Reactivity ：Human
Reported Reactivity ：African Green, Baboon, Cynomolgus, Rhesus
Antibody Type ：Monoclonal
Host Species ：Mouse
Immunogen ：BAFF-R-L1.2 transfectants
Formulation ：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation ：The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 underoptimal conditions.
Concentration ：Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysis online tool.)
Storage & Handling ：The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application ：FC - Quality tested
Recommended Usage ：Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in100 µl staining volume or 5 µl per 100 µl of whole blood.
* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.
Excitation Laser ：Blue Laser (488 nm)、Green Laser (532 nm)、Yellow-Green Laser (561 nm)
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文献和实验1. Ng LG, et al.2004. J. Immunol. 173:807. (FC IHC)
2. Personal communication. (Block)
Product Citations：
1. Dutertre CA, et al. 2020. Immunity. 51(3):573-589.e8.. PubMed
度的功能细胞。二、人外周血中 CD4 T 细胞分选实例1.产品信息： MojoSort™ Human CD4 T Cell Isolation Kit（BioLegend, Cat#480009），阴性分选试剂盒2.检测仪器型号：BD LSRⅡ3.样本：正常人外周血4.分选前后检测相关产品：PE-CD4（Biolegend, Cat# 317410）5.实验过程（1）采血之后采用达优™ 人淋巴细胞分离液分离人外周血单个核细胞。分离之后能见到明显的白膜层。（达优，Cat#DKW-KLSH-0100
R&D Systems:如何提高MSC的扩增且不损失其分化潜能
- M E M 培养基（Invitrogen，Carlsbad）。将1.25×105量级的hMSCs接种于一个T75长颈瓶，在细胞汇聚之前让细胞生长3至4天。在培养期末，培养于不同条件的hMSCs同时用胰蛋白酶/EDTA溶液收集。对细胞计数，并且用下面的公式计算扩增倍数。重新培养时，将1.25×105量级的hMSCs接种于一个新的T75长颈瓶，并用适当的培养基培养。每次传代后对细胞进行计数。 抗体。藻红蛋白（PE）连接的小鼠抗人CD90和CD105的单克隆抗体（mAbs），PE非连接的山
5.5, APC等。 常见荧光素波长分布示意图 同型对照的选择： 流式细胞实验和其他抗体相关实验有点不同的就是需要选择同型对照。同型对照，是用于消除由于抗体非特异性结合到细胞表面而产生的背景染色，相当于实验的阴性对照。同型对照选择与标记抗体同种属来源，同亚型，同荧光标记的抗体。比如：抗人的CD3的PE标记的抗体，小鼠的IgG2a。同型
