相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
999
- 靶点:
CD70
- 级别:
CD70 Antibody
- 目录编号:
CD70 Antibody
- 克隆性:
单克隆
- 抗原来源:
小鼠
- 保质期:
CD70 Antibody
- 抗体英文名:
Fire™ 750 anti-human CD70 Antibody-APC
- 抗体名:
CD70 抗体
- 标记物:
APC
- 宿主:
小鼠
- 适应物种:
人
- 免疫原:
CD70 Antibody
- 亚型:
CD70 Antibody
- 形态:
CD70 Antibody
- 应用范围:
FC
- 保存条件:
2-8℃
- 浓度:
CD70 Antibody
- 规格:
25 tests/100 tests
| 规格: | 25 tests | 产品价格: | ¥3000.0 |
|---|---|---|---|
| 规格: | 100 tests | 产品价格: | ¥6500.0 |
Description :CD70, also known as CD27L, is a 50 kD type II transmembrane glycoprotein and member ofthe tumor necrosis factor superfamily. CD70 is expressed on activated T, B and NK cells,activated plasmacytoid dendritic cells (pDCs), and chronic B cell lymphocytic leukemia andlarge B cell lymphomas. CD70 costimulates T cell proliferation and differentiation. It plays a rolein the pDC-induced B cell differentiation. The ligand of CD70 is CD27.
Verified Reactivity :Human
Antibody Type :Monoclonal
Host Species :Mouse
Immunogen :CD70-transfected L cells
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation :The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 underoptimal conditions.
Concentration :Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysis online tool.)
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application :FC - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in100 µL staining volume or 5 µL per 100 µL of whole blood.
* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm
Excitation Laser :Red Laser (633 nm)
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验1. Shaw J, et al. 2010. Blood 115:3051. (Block)
抗体二抗上连接有荧光染料。直接标记染色背景低,实验程序少尽量减少实验工序和过程,以保证实验的真实和准确性。因此在条件允许的范围内,建议尽量用直接标记的抗体进行实验。 3、流式抗体荧光标记的选择: 如果实验中检测单一指标:不同荧光标记在不同的仪器上强度不同。以某仪器为例:PE >APC >PE-Cy5 >PerCP >FITC >PerCP-Cy5.5,通常来说,PE最强,适用于弱表达抗原。FITC强度较弱,适用于强表达抗原,使用范围比较广。用户需根据检测的目标蛋白进行具体选择。 如果同时检测多个
焦显微镜,单分子成像等条件要求苛刻的应用中。CF 750、CF?770 andCF?790等近红外荧光染料;CF750、CF770、CF790是领域内真正具有突破性的长波长的新型染料代表。其他近红外染由于受到染料聚集和稳定性差等因素的影响而导致荧光亮度有限。基于Biotium 科学家研制的新型分子工程技术,近红外CF 染料克服了这些问题。即使在633 nm 被激发,CF750也会比APC-AlexaFluor 750 拥有足够的亮度来在流式细胞仪检测中带来更好的信噪比,而且不存在串联染料的低稳定性等问题
5.5, APC等。 常见荧光素波长分布示意图 同型对照的选择: 流式细胞实验和其他抗体相关实验有点不同的就是需要选择同型对照。同型对照,是用于消除由于抗体非特异性结合到细胞表面而产生的背景染色,相当于实验的阴性对照。同型对照选择与标记抗体同种属来源,同亚型,同荧光标记的抗体。比如:抗人的CD3的PE标记的抗体,小鼠的IgG2a。同型










