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文献和实验Isolation and Culture of Mouse Germ Cells
cells have subsequently been found to be physiologically relevant to germ cell growth and development in vivo. For example, leukemia inhibitory factor (LIF) was identified as a potent germ cell survival factor and mitogen in culture (1 ). Subsequently
Deriving and Propagating Mouse Embryonic Stem Cell Lines for Studying Genomic Imprinting
of division in the undifferentiated state by virtue of the action of exogenous factors. Leukemia inhibitory factor, LIF, is one such factor (2,3) and is indispensable for the propagation of mouse ES cells at least when primary embryo fibroblasts (PEFs
Feeder-Independent Culture of Mouse Embryonic Stem Cells Using Vitamin A/Retinol
inhibitory factor (LIF) which is supplemented with 0.5 μM retinol/vitamin A. The cells are passaged every 3–5 days by trypsinization. The pluripotency of the cells is tested by different undifferentiated ES cell-specific markers.
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