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文献和实验Isolation and Culture of Mouse Germ Cells
cells have subsequently been found to be physiologically relevant to germ cell growth and development in vivo. For example, leukemia inhibitory factor (LIF) was identified as a potent germ cell survival factor and mitogen in culture (1 ). Subsequently
Neural Differentiation of Embryonic Stem Cells
of leukemia inhibitory factor (LIF). ES cells appear to have unlimited proliferative capability, and, remarkably, when returned to the inner cell mass after culture and gene manipulation, resume their development and participate fully in the formation
Feeder-Independent Culture of Mouse Embryonic Stem Cells Using Vitamin A/Retinol
inhibitory factor (LIF) which is supplemented with 0.5 μM retinol/vitamin A. The cells are passaged every 3–5 days by trypsinization. The pluripotency of the cells is tested by different undifferentiated ES cell-specific markers.
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