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文献和实验were passed through 1-ml nickel affinity columns (B-PER 6×His fusion protein purification kit from Pierce)that had been pre-equilibrated with 7 M GuHCl in TBS (0.15 M NaCl,50 mM Tris.HCl pH 7.5).The columns were washed thrice with 1 ml 7 M GuHCl
Polyclonal Antibody Production 简单快捷制备多抗方法,可用于western、ELISA和免疫/b
Very useful for rapid and simple generation of antibodies for western blots, ELISA assays, and immunoprecipitation. -------------------------------------------------------------------------------- Rabbit
Searching for Non‐B DNA‐Forming Motifs Using nBMST (Non‐B DNA Motif Search Tool)
conformations, genomic rearrangements, and human disease. J. Biol. Chem. 279:47411‐47414. Bacolla, A. and Wells, R.D. 2009. Non‐B DNA conformations as determinants of mutagenesis
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