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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CREB Regulated Transcription Coactivator 1 (CRTC1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CREB Regulated Transcription Coactivator 1 (CRTC1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Intra-Assay: CV<10%. Inter-Assay: CV<12%
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文献和实验相关实验
VE-1 binding sites (comprising the 9 bp putative interacting sequence and three flanking base pairs) were determined by ELISA and normalized to VE-1 subs. The positions of the potential binding sites relative to the transcription start site
酰转移酶基因和去乙酰化酶基因均已被克隆。P/CAF为人类中被克隆的乙酰转移酶基因，研究表明, P/CAF基因产物与已知的转录共激活子(coactivator)CBP和p300结合, 后者与转录因子（例如CREB, c-JUN和FOS)结合, 因此, Sternglanz(1996)等〔26〕认为, 乙酰转移酶通过与转录因子和共激活子结合, 催化启动子区核心组蛋白的乙酰化。 5 核小体与基因调控 核小体是染色质的基本结构单位。体内外实验均证实, 核小体为基因转录的一个通用抑制子(general
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Mouse CREB Regulated Transcription Coactivator 1 (CRTC1) ELISA Kit
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