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文献和实验Flow Cytometric Measurement of SLAM-Associated Protein and X-Linked Inhibitor of Apoptosis
of immune homeostasis, SLAM-associated protein (SAP) and X-linked inhibitor of apoptosis (XIAP). These proteins are defective in patients with the immune deficiency X-linked lymphoproliferative disease (XLP), due to mutations in the SH2D1A and XIAP/BIRC
. Using this technique, we can detect proteins such as X-linked inhibitor of apoptosis protein (XIAP) and Fas ligand (FasL) with as little as 1000 cells.
Native Chromatin Preparation and Illumina/Solexa Library Construction
to chromatin as histones, the chromatin should be cross-linked using formaldehyde and sonicated to 200- to 300-bp length. 22. Resuspend 20-22 x 106 purified human CD4+ T cells from Step 21 in 1 mL MNase digestion buffer kept at room
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