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文献和实验Sex Determination by PCR Analysis of the X-Y Amelogenin Gene
, but it is impossible to tell whether the template DNA is from a female or whether the analysis has ended in failure when no fragment is amplified by PCR. Simultaneous amplification of X- and Y-chromosomal genes is thus necessary for a reliable sex test, in which the X
We present a fluorogenic real-time polymerase chain reaction (PCR) procedure to target a segment (106-112 base pairs [bp]) of the X-Y homologous amelogenin gene by measuring the 5′' nuclease activity of the Taq deoxyribonucleic acid (DNA
Clonality - X Chromosome Inactivation Assay
and can assist in interpretation of allele patterns. 4. References Allen RC, Zoghbi HY, Moseley AB, Rosenblatt HM, Belmont JW. Methylation of HpaII and HhaI sites near the polymorphic CAG repeat in the human androgen-receptor gene correlates with X chromosome
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