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文献和实验with fetal calf serum (FCS). However, serum contains a wide variety of biologically active compounds that might adversely affect hESC growth and differentiation. Thus, cultivation of stem cells in FCS complicates experimental approaches to define
Isolation, Purification, and Growth of Human Skeletal Muscle Cells
of myoblasts from human muscle tissue using two different techinques flow cytometry (11 ) and cell cloning (12 –14 ). These methods are applicable to muscle tissue from both fetal and postnatal donors, as well as from normal and diseased individuals
Human Embryonic Stem Cells: Isolation, Maintenance, and Differentiation
diverse lineages are developing. Furthermore, strategies using specific growth factor combinations, cell-cell and cell-extracellular matrix induction systems are being explored for directed differentiation along a desired lineage. However, there is a great
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