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文献和实验Assay of G Protein-Coupled Receptor Kinase Activity by Rhodopsin Phosphorylation
Agonist occupation of G protein-coupled receptors (GPCRs) leads to a cellular response that wanes, or desensitizes, with prolonged agonist exposure. Different cellular mechanisms contribute to desensitization, including the altered function
Discovery and Function of the Very Large G Protein-Coupled Receptor
The very large G protein-coupled receptor 1 (VLGR1), also known as MASS1 or GPR98, is most notable among the family of adhesion GPCR for its size. Encoded by an 18.9 kb open reading frame, the ~700 kDa primary translation product is by far
Elucidating Agonist-Selective Mechanisms of G Protein-Coupled Receptor Desensitization
(PKC) and G protein-coupled receptor kinase/arrestin respectively. This could explain why opioid agonists have variable clinical profiles and liabilities to induce tolerance and dependence. Here we describe the experimental approaches
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