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文献和实验Purification of Myosin Light Chain Kinase
RPM (4℃).Filter the supernatant through glass wool and discard the pellet (or save for light chain prep).Save 1 ml of supernatant (4℃)for assay.Add pepstatin and TLCK to final concentrations of 1 μg/ml and 40 Og/ml,respectively. Volume of supernatant
Isolation and Culture of Human Airway Smooth Muscle Cells
doublings and express smooth muscle cell-specific markers such as myosin heavy chain, myosin light chain kinase, α-actin, caldesmon, and SM22α . In addition, the synthetic function of these cells appears to be comparable to those studied in vivo
Screening cDNA Libraries Using Partial Probes to Isolate Full-Length cDNAs from Vascular Cells
within the library selected (1 ,2 ). As an example of both the utility and power of library screening, we will relate our own library screening efforts utilized to isolate the nonmuscle high molecular weight myosin light chain kinase isoform from a human umbilical
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