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文献和实验The Polymerase Chain Reaction (PCR)
Topics • What is the Polymerase Chain Reaction? • History and (pre-history) of PCR • How PCR works • Optimising PCR • Fidelity, errors and cloning • PCR primer design • Applications of PCR What is the Polymerase Chain Reaction? • It’s
Single Primer ("Semi-Random") P
% of the time in our lab for any given primer Po (see figure below) It has been adapted from Hermann et al (1). The "Long" method, developed in our laboratory (2), requires more time but will often work when the short method fails. Another advantage of the long
Rapid Extraction of High Quality DNA from Whole Blood Stored at 4ºC for Long Period
100. Add 10 ml of 1 M Tris, 109.54 gr of sucrose, 1.01 gr MgC12, adjust pH to 8.0 and finally add 10 ml of Triton X-100 to 800 ml of distilled water, and make up to 1 liter with distilled water. Autoclave at 15 p.s.i. for 10 min. Sugars at high
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