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文献和实验Myosin Light Chain Preparation
) For Cardiac Muscle- (1.5 liters ETOH 95% (1.5 liters H2O,3 ml 15 M MCE) Buffer E.Column Buffer: 16 liters: 20 mM Tris,15 mM MCE (160 ml 2 M Tris,16 ml 15 M MCE) Buffer F.Elution Buffer: 4 liters: 0.150 M KCl,10 mM Tris,15 mM MCE(150 ml 4 M KCl,40 ml 2 M
Purification of Myosin Light Chain Kinase
Solutions 1.1 M Acetic Acid 2.Buffer A Homogenization 10 mM MOPS- 40 ml of 2M stock solution 8 liters 4 mM EDTA- 160ml of 0.2M stock solution 1 mM DTT* - 8ml of 1M stock solution 0.1 mM PMSF* - 14ml of 10mg/ml stock solution 10 μg/ml trypsin
Isolation and Culture of Human Airway Smooth Muscle Cells
doublings and express smooth muscle cell-specific markers such as myosin heavy chain, myosin light chain kinase, α-actin, caldesmon, and SM22α . In addition, the synthetic function of these cells appears to be comparable to those studied in vivo
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