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文献和实验Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
a 200 μL pipette, transfer the cut pieces to a freshly prepared 24-well MEF culture plate containing human iPSC medium. 6) Incubate the MEF culture plate containing the picked colonies in a 37°C incubator with a humidified atmosphere of 5% CO2
Human IL-2 Enzyme Immunoassay (EIA)
-2 EIA is an in vitro enzyme-linked immunosorbent assay for quantitatively detecting human IL-2 in biological fluids (e.g. serum, plasma) and in cell culture supernatants. The kit consists of a 96 well microtiter plate that has been precoated with anti-IL
Activation and Expansion of Human Treg Cells
from the magnet. (5)Resuspend the washed Dynabeads in the same volume of Buffer 1 as the initial volume of Dynabeads. 2. Isolation of Human Treg Cells For isolation of human Treg cells it is recommended to use the Dynal CD4 CD25 Treg Kit. 3. Expansion
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