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文献和实验The Role of Calpain in Neurofilament Protein Degradation Associated With Spinal Cord Injury
proteins (NFPs), vimentin, micro-tubule-associated protein (MAP), and myelin basic protein (MBP) are known to be calpain substrates. The μ-and m-calpains also cleave protein kinase C (PKC), hormones, receptors, and histones (3 ).
. Their activities can be measured experimentally with kinase assays that use specific exogenous substrates. The activities of MPF and MAPK are measured using histone H1 kinase and MBP (Myelin Basic Protein) kinase assays, respectively
our extensive experience with B-PER for isolating fusion proteins in which the fusion partner is either IPIII-His (an internal protein of bacteriophage T4 with a His tag at the C-terminus)or MBP (the E.coli maltose binding protein).The two cases illustrate
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