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文献和实验ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
is available in which the albumin binding site has been deleted. Agarose beads coated with these proteins will bind antibodies and serve to immunoprecipitate antigens from solution. There are differences in the affinity of these proteins for antibodies
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
, such as the interaction with other partner proteins. For example, a transgene construct containing a cis -regulatory DNA element may create a new protein-binding site at the integration site on a chromosome (Fig. 2 ). View larger version
our extensive experience with B-PER for isolating fusion proteins in which the fusion partner is either IPIII-His (an internal protein of bacteriophage T4 with a His tag at the C-terminus)or MBP (the E.coli maltose binding protein).The two cases illustrate
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