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- 2025年07月11日
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文献和实验Generation of Mouse Bone Marrow-Derived Macrophages
of M-CSF, the macrophage colony stimulating factor, and it is characterised by high yield and reproducibility. Once obtained, bone marrow-derived macrophages (BMMs) can be used for a considerable number of functional and structural assays and are commonly
Osteoclast Formation in the Mouse Coculture Assay
) to stimulate RANKL and macrophage colony-stimulating factor (M-CSF) expression. These factors then stimulate early osteoclast precursors present in the spleen or bone marrow cell populations to differentiate into mature osteoclasts. At the end of the culture
Isolation and Propagation of Mouse Liver-Derived Dendritic Cells
, these lymphoid-derived DC can be generated in the absence of granulocyte macrophage colony stimulating factor (GM-CSF), and are CD8+ and Fas ligand+ (CD95L+ ). In the presence of GM-CSF, DC develop directly from myeloid committed precursors, which also give rise
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