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文献和实验Arthritis Induced with Minor Cartilage Proteins
Type XI collagen (CXI) and cartilage oligomeric matrix protein (COMP) are minor components in cartilage, shown to be arthritogenic. CXI is a heterotrimeric triple helical fibrillar collagen and intermingled in the collagen fibers with type II
Human Chondrocyte Cultures as Models of Cartilage-Specific Gene Regulation
Cartilage oligomeric matrix protein (COMP; thrombospondin-5) Thrombospondin-1 and -3 Cartilage matrix protein (matrilin-1); matrilin-3
at 4°C for 15 minutes. 9. Remove and aliquot supernatant. We recommend making several 50 μl aliquots. 10. Before running ELISA, dilute protein 1:100 and run a Bradford total protein assay or Quant-iT™ Protein Quantitation Kit assay (Cat. no. Q
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