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文献和实验Characterizing Bile Acid and Lipid Metabolism in the Liver and Gastrointestinal Tract of Mice
system under both normal and pathological conditions. Methods are presented for characterizing liver and intestinal function with a focus on bile acid and lipid metabolism in the gut?liver axis. Curr. Protoc. Mouse Biol. 1:289?321 © 2011 by John Wiley & Sons
of chemical liver injury or surgical resection that faithfully recapitulate hallmarks of human pathophysiology and trigger robust hepatocyte proliferation leading to organ restoration. The advent of mouse transgenics has undeniably catalyzed the wider
在RT-PCR中避免DNA污染(Avoiding DNA Contamination in R
present in total RNA samples isolated by most commonly used techniques. To illustrate this problem, we performed RT-PCR on mouse liver RNA isolated by a multi-step guanidinium thiocyanate/acid phenol:chloroform extraction (ToTALLY RNA™), a one-step
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