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文献和实验Analysis of Osteoclast Function in Mouse Calvarial Cultures
can produce pits (8 ), and two distinct morphologies of multinucleate tartrate-resistant acid phosphatase (TRAP)-positive osteoclast-like cells have been described with differing resorptive capacities (9 ). Like the osteoclast, the foreign body giant cell
for primary antibody), labeled with alkaline phosphatase or horseradish peroxidase. Substrate appropriate to the enzyme conjugate (HRP or AP). Appropriate wash buffer: Phosphate-buffered saline (PBST): 10 mM sodium phosphate, pH 7.2, 0.9% (w/v
【申请上传】Current Protocols in Immunology
Immunocompromised Animals Unit 1.3 Handling and Restraint Unit 1.4 Anesthesia Unit 1.5 Animal Identification Section II Use of Mouse, Rat, Hamster, and Rabbit Unit 1.6 Parenteral Injections Unit 1.7 Blood Collection Unit 1.8 Euthanasia Section III Selected
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