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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
999
- 靶点:
IgD
- 级别:
anti-mouse IgD Antibody
- 目录编号:
anti-mouse IgD Antibody
- 克隆性:
单克隆
- 抗原来源:
大鼠
- 保质期:
anti-mouse IgD Antibody
- 抗体英文名:
Alexa Fluor® 700 anti-mouse IgD Antibody
- 抗体名:
IgD 抗体
- 标记物:
anti-mouse IgD Antibody
- 宿主:
大鼠
- 适应物种:
小鼠
- 免疫原:
anti-mouse IgD Antibody
- 亚型:
anti-mouse IgD Antibody
- 形态:
anti-mouse IgD Antibody
- 应用范围:
FC
- 保存条件:
2-8℃
- 浓度:
0.5 mg/ml
- 规格:
25 ug/100 ug
| 规格: | 25 ug | 产品价格: | ¥2100.0 |
|---|---|---|---|
| 规格: | 100 ug | 产品价格: | ¥5000.0 |
Description :Surface IgD is an important B cell differentiation marker.
Verified Reactivity :Mouse
Antibody Type :Monoclonal
Host Species :Rat
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation :The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700under optimal conditions.
Concentration :0.5 mg/ml
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application :FC - Quality tested、SB - Reported in the literature, not verified in house
Recommended Usage :Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cellsin 100 µl volume. It is recommended that the reagent be titrated for optimal performance for eachapplication.
* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Priorto using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flowcytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
Excitation Laser :Red Laser (633 nm)
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文献和实验1. Nitschke L, et al. 1993. P. Natl. Acad. Sci. USA 90:1887. (FC)
2. Weih D, et al. 2001. J. Immunol. 167:1909. (IHC)
3. Koni PA, et al. 2001. J. Exp. Med. 193:741. (IHC)
4. Ahuja A, et al. 2007. J. Immunol. 179:3351. (FC) PubMed
5. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
6. Good-Jacobson KL, et al. 2010. Nat. Immunol. 11:535. (FC) PubMed
7. Tomayko MM, et al. 2010. J. Immunol. 185:7146. PubMed
8. Park SY, et al. 2013. J. Immunol. 190:1094. PubMed
9. Rouaud P, et al. 2014. J Exp Med. 211:975. PubMed
10. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
11. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations:
1. Pani F, et al. 2021. Endocrinology. 162:. PubMed
2. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
3. Vijayan K, et al. 2021. Cell Reports. 36(5):109489. PubMed
4. Yen WF et al. 2019. Cell reports. 27(5):1472-1486 . PubMed
5. Yewdell WT, et al. 2020. Immunity. 53(5):952-970.e11. PubMed
6. Wilder BK, et al. 2022. NPJ Vaccines. 7:58. PubMed
7. Zhu H, et al. 2019. Nat Commun. 10:1084. PubMed
8. Staffas A et al. 2018. Cell host & microbe. 23(4):447-457 . PubMed
9. Zhong C, et al. 2021. J Virol. 95:e0092521. PubMed
10. Carbonetti S et al. 2017. Journal of immunological methods. 448:66-73 . PubMed
11. Song W, et al. 2022. Immunity. 55:290. PubMed
常用抗体标记荧光染料的选择 随着免疫荧光技术的不断发展,荧光染料及其标记的抗体偶联物也被广泛的应用于生物学实验中。目前,市场上抗体及蛋白标记的荧光染料主要有CFTM系列(BIOTIUM, USA); Alexa Fluor®系列 (Life technology, USA); DyLight系列;Cy系列; IR Dye系列等等。使用最多的为AlexaFluor®系列和CFTM系列。CFTM系列染料的核心对比AlexaFluor®系列具有以下几点优势:1. 新型罗丹明核心罗丹明染料以优异
随着免疫荧光技术的不断发展,荧光染料及其标记的抗体偶联物也被广泛的应用于生物学实验中。目前,市场上抗体及蛋白标记的荧光染料主要有CFTM系列(BIOTIUM, USA); Alexa Fluor®系列(Life technology, USA); DyLight系列; Cy系列; IR Dye系列等等。使用最多的为Alexa Fluor®系列和CFTM系列。 一、CFTM系列染料的核心对比Alexa Fluor®系列具有以下几点优势: 1、新型罗丹明核心 罗丹明染料以优异
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种类型的荧光抗体在单个标本中准确展示荧光标记分子的共定位情况。 PLAPON60XOSC 和 UPLSAPO60XO 的性能比较 2. 超级色差校正物镜的应用-脑组织的四重免疫荧光 图 1. 脑组织的四重免疫荧光。 VIAAT 染色(Alexa Fluor405,蓝色);CB1 染色(Alexa Fluor488,绿色);VGluT3 染色(Cy3,红色);DGLα染色(Alexa Fluor647,白色)。 大麻素合成酶 DGLα(白色)和大麻素受体 CB1(绿色)聚集在小鼠











