Description :Surface IgD is an important B cell differentiation marker
Verified Reactivity :Mouse
Antibody Type :Monoclonal
Host Species :Rat
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation :The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647under optimal conditions.
Concentration :0.5 mg/mL
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Recommended Usage :Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per millioncells in 100 µL volume. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance foreach application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
Excitation Laser :Red Laser (633 nm)
Application Notes The 11-26c.2a antibody reacts with immunoglobulin D in all tested mouse haplotypes. The antibodybinds membrane IgD expressed on most B cells. The 11-26c.2a antibody neither inducesproliferation of splenic B cells nor induces B cell activation. Additional reported applications (for therelevant formats) include: immunohistochemical staining of a-cetone-fixed frozen sections , andspatial biology (IBEX) 10,11.
Application References(PubMed link indicatesBioLegend citation):
1. Nitschke L, et al. 1993. P. Natl. Acad. Sci. USA 90:1887. (FC)
2. Weih D, et al. 2001. J. Immunol. 167:1909. (IHC)
3. Koni PA, et al. 2001. J. Exp. Med. 193:741. (IHC)
4. Ahuja A, et al. 2007. J. Immunol. 179:3351. (FC) PubMed
5. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
6. Good-Jacobson KL, et al. 2010. Nat. Immunol. 11:535. (FC) PubMed
7. Tomayko MM, et al. 2010. J. Immunol. 185:7146. PubMed
8. Park SY, et al. 2013. J. Immunol. 190:1094. PubMed
9. Rouaud P, et al. 2014. J Exp Med. 211:975. PubMed
10. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
11. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations:
1. Soon MSF, et al. 2020. Nat Immunol. 1.984027778. PubMed
2. Chan T, et al. 2009. J Immunol. 183:3139. PubMed
3. Vanderleyden I, et al. 2020. Cell Rep. 30:611. PubMed
4. Wang L, et al. 2019. Cell Rep. 29:1848. PubMed
5. Gallman AE, et al. 2021. Sci Immunol. 6:. PubMed
6. Rodda LB et al. 2018. Immunity. 48(5):1014-1028 . PubMed
7. Shi B, et al. 2018. J Immunol. 200:586. PubMed
8. Lindner J, et al. 2013. Mol Cell Biol. 33:4628. PubMed
9. Gatto D, et al. 2011. J Immunol. 187:4621. PubMed
10. Vu T, et al. 2011. J Immunol. 187:5032. PubMed
11. Biram A, et al. 2020. Cell Rep. 30:1910. PubMed
12. Adori M, et al. 2018. J Immunol. 200:775. PubMed