Brilliant Violet 421™ 抗小鼠 CD27

Brilliant Violet 421™ anti-mouse CD274 (B7-H1, PD-L1) Antibody详细如下：

Description ：CD274, also known as B7-H1 or programmed death ligand 1 (PD-L1), is a 40 kD type Itransmembrane protein and a member of the B7 family within the immunoglobulin receptorsuperfamily. It is expressed on T cells, B cells, NK cells, dendritic cells, IFN-γ activatedendothelial cells, and monocytes. B7-H1 is one of the ligands of PD-1. The interaction of B7-H1with PD-1 plays an important role in the inhibition of T cell responses. Other studies haveshown that B7-H1 is able to costimulate T cell growth and cytokine production. CD274 isinvolved in costimulation essential for T cell proliferation and production of IL-10 and IFN-γ, inan IL-2-dependent and a PD-1-independent manner. Its interaction with PD-1 inhibits T cellproliferation and cytokine production.

Verified Reactivity ：Mouse
Antibody Type ：Monoclonal
Host Species ：Rat
Formulation ：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation ：The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™under optimal conditions.
Concentration ：Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysis online tool.)
Storage & Handling ：The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.

Application ：FC - Quality tested

Recommended Usage ：Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent betitrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.

Excitation Laser ：Violet Laser (405 nm)