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- 齐源
- 656804
- 国产/进口
- 2025年07月12日
- ICC, IHC-P
- 小鼠
- 人
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
齐源
- 库存：
999
- 靶点：
巢蛋白
- 级别：
anti-Nestin Antibody
- 目录编号：
anti-Nestin Antibody
- 克隆性：
单克隆
- 抗原来源：
小鼠
- 保质期：
anti-Nestin Antibody
- 抗体英文名：
Alexa Fluor® 594 anti-Nestin Antibody
- 抗体名：
巢蛋白抗体
- 标记物：
anti-Nestin Antibody
- 宿主：
小鼠
- 适应物种：
人
- 免疫原：
anti-Nestin Antibody
- 亚型：
anti-Nestin Antibody
- 形态：
anti-Nestin Antibody
- 应用范围：
ICC, IHC-P
- 保存条件：
2-8℃
- 浓度：
0.5 mg/ml
- 规格：
100 ug
Description ：Nestin is a class VI intermediate filament protein originally found in neuronal stem cells, and isrequired for survival and renewal of neural progenitor cells. In mixtures, Nestin formsheterodimers with vimentin or internexin, participating in the remodeling of the cell. Nestin wasfound to be expressed on vascular endothelial progenitor cells and various neoplasms. It isproposed to be a novel angiogenesis marker and cancer therapeutic target.
Verified Reactivity ：Human
Antibody Type ：Monoclonal
Host Species ：Mouse
Immunogen ：Fusion protein
Formulation ：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation ：The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594under optimal conditions.
Concentration ：0.5 mg/ml
Storage & Handling ：The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application ：ICC - Quality tested
IHC-P - Verified
Recommended Usage ：Each lot of this antibody is quality control tested by immunocytochemistry. Forimmunocytochemistry, a concentration range of 1.0 - 5.0 µg/ml is recommended. Forimmunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommendedthat the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
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文献和实验1. Jozef S, et al. 2012. Biomed. Pap. Med. Fac. 156:135. (IHC)
保持优异的水溶性，为该波段最亮的橙色荧光染料。例如：同等的标记程度下，CF543 标记的羊抗鼠IgG抗体的亮度高于用Alexa Fluor 546 标记的2~10倍。CF 555、AlexaFluor 555、Tetramethylrhodamine(TAMRA) 等匹配Cy3滤光片的橙色荧光染料；红色（568-594nm处激发）CF 568、Alexa Fluor® 568,ATTO 565, Rhodamine Red等568nm处红色荧光染料；CF 568染料耐光性最好；高效水溶
，耐光，水溶性好，确保了CF 543染料与抗体的耦联物保持优异的水溶性，为该波段最亮的橙色荧光染料。例如：同等的标记程度下，CF 543标记的羊抗鼠IgG抗体的亮度高于用Alexa Fluor 546 标记的2~10倍。 CF 555、Alexa Fluor 555、Tetramethylrhodamine (TAMRA) 等匹配Cy3滤光片的橙色荧光染料。 4、红色（568-594nm处激发） CF 568、Alexa Fluor® 568, ATTO 565
，Alexa Fluor 488）和γH2AX（红色，Alexa Fluor 594）。通道合并图像显示 CDH4 和γH2AX 的共定位。
