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- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
999
- 靶点:
CD117
- 级别:
anti-human CD117 (c-kit) Antibody
- 目录编号:
anti-human CD117 (c-kit) Antibody
- 克隆性:
单克隆
- 抗原来源:
小鼠
- 保质期:
anti-human CD117 (c-kit) Antibody
- 抗体英文名:
Alexa Fluor® 647 anti-human CD117 (c-kit) Antibody
- 抗体名:
CD117 Antibody
- 标记物:
anti-human CD117 (c-kit) Antibody
- 宿主:
小鼠
- 适应物种:
人
- 免疫原:
anti-human CD117 (c-kit) Antibody
- 亚型:
anti-human CD117 (c-kit) Antibody
- 形态:
anti-human CD117 (c-kit) Antibody
- 应用范围:
FC
- 保存条件:
2-8℃
- 浓度:
anti-human CD117 (c-kit) Antibody
- 规格:
25 tests/100 tests
| 规格: | 25 tests | 产品价格: | ¥2600.0 |
|---|---|---|---|
| 规格: | 100 tests | 产品价格: | ¥5500.0 |
Description :CD117 is a 145 kD protein tyrosine kinase also known as c-Kit. It is a receptor for stem cellfactor or c-Kit ligand. CD117 is expressed on pluripotent hematopoietic progenitor cells(approximately 1-4% bone marrow cells), mast cells, and acute myeloid leukemia cells (AML).CD117 binding of c-Kit ligand induces phosphorylation of CD117 and stimulates proliferationand survival of primitive hematopoietic stem cells as well as erythroid-committed and granulomonocytic committed cells.
Verified Reactivity :Human
Reported Reactivity :Cynomolgus, Cow
Antibody Type :Monoclonal
Host Species :Mouse
Immunogen :MOLM-1 megakaryocytic cell line
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation :The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647under optimal conditions
Concentration :Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysis online tool.)
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application :FC - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in100 µl staining volume or 5 µl per 100 µl of whole blood.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
Excitation Laser :Red Laser (633 nm)
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文献和实验1. Broudy VC, et al. 1999. Blood 94:1979. (IF, IP)
2. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
3. Nagano M, et al. 2007. Blood 110:151. (FC) PubMed
4. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
5. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
免疫表型策略。首先,所有ILCs均被定义为CD45阳性且系别阴性(Lin-),其中Lin-排除了T细胞、B细胞、髓系细胞等其他免疫谱系。在此基础上,通过关键表面标志物区分各亚群:NK细胞表达CD94及不同强度的CD56;而辅助性ILC1、ILC2和ILC3均为CD94阴性但表达CD127。进一步区分这三类细胞依赖于CD117(c-kit)和CRTH2的表达差异:ILC1为双阴性,ILC2通常为CRTH2阳性,而ILC3则为CD117阳性且CRTH2阴性。这一组合标记法是精准分离和研究各类ILCs
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换液会导致细胞漂浮。 Elabscience®相关产品推荐 货号 产品名称 E-CK-A103 人外周血单个核细胞分离液(P 1.077) E-AB-F1137C FITC 标记抗人 CD45 抗体 [HI30] E-AB-F1081D PE 标记抗小鼠/人/猴 CD11b 抗体 [M1/70] AN00213Q Elab Fluor® Violet 450 标记抗人 CD14 抗体 [H332-1B10] E-AB-F1299M Elab Fluor® 647
一、如何搭配流式抗体荧光标记 流式抗体荧光标记搭配主要由3个因素决定的:流式细胞仪能检测多少个通道、需要同时检测检测多少个指标、厂家的抗体有多少种荧光标记。如果流式细胞仪的通道越多,那么同一份样本能同时做的表面/胞内标志就越多 A、 如何根据流式细胞仪搭配流式抗体 1) BD流式细胞仪(06版目录第614页) 流式细胞仪能检测多少个通道是由有多少根激光决定的以及每根激光
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