BioLegend 947004 Ultra-LEAF™ P

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BioLegend 947004 Ultra-LEAF™ Purified anti-mouse CCL5 (RANTES)
产品简介：
产品品牌：BioLegend
产品货号：947004
产品名称：BioLegend 947004 Ultra-LEAF™ Purified anti-mouse CCL5 (RANTES)
产品规格：1 mg

产品说明：
Clone   W20042A (See other available formats)
Regulatory Status  RUO
Other Names
SIS-delta, T-cell specific protein p288, Regulated on Activation Normal T-expressed and Secreted (RANTES), Wmall inducible cytokine A5
Isotype   Rat IgG2a, λ

描述
CCL5是一种CC趋化因子，在免疫和炎症反应中指导白细胞的迁移。CCL5表达的增加与广泛的炎症性疾病和病理学有关。已经表明CCL5在原发肿瘤部位由乳腺肿瘤细胞高度表达，并且与乳腺癌症的进展密切相关。CCL5可能通过将单核细胞募集到肿瘤部位来促进肿瘤进展，CCL5还可以刺激MMP的释放，从而促进肿瘤细胞迁移。一些研究还表明，CCL5在体内和体外具有促血管生成的作用。CCL5寡聚化和与GAGs结合可以通过其受体CCR1和CCR5诱导血管生成作用。CCL5在许多病毒感染的早期阶段表达。已经表明CCL5对许多病毒具有保护作用，包括流感病毒、呼吸道合胞病毒、HCMV、HCV和HIV。CCL5可以通过与病毒竞争CCR5结合来抑制HIV感染。CCL5也与哮喘、动脉粥样硬化和纤维化的发展有关。
CCL5 is a CC chemokine that directs the migration of leukocytes in immune and inflammatory responses. Increased CCL5 expression has been associated with a wide range of inflammatory disorders and pathologies. It has been shown that CCL5 is highly expressed by breast tumor cells at primary tumor sites and is strongly associated with the progression of breast cancer. CCL5 may contribute to tumor progression by recruiting monocytes into tumor sites, and CCL5 can also stimulate the release of MMP, thereby promoting tumor cell migration. Some studies also show that CCL5 has pro-angiogenesis effects in vivo and in vitro. CCL5 oligomerization and binding to GAGs can induce angiogenic effects through its receptor CCR1 and CCR5. CCL5 is expressed in the early stages of many viral infections. It has been shown that CCL5 is protective against many viruses including influenza, RSV, HCMV, HCV, and HIV. CCL5 can inhibit HIV infection by competing with the virus for CCR5 binding. CCL5 also has been implicated in the development of asthma, artherosclerosis, and fibrosis.

Product Details
Verified Reactivity  Mouse
Antibody Type  Monoclonal
Host Species  Rat
Immunogen  Recombinant mouse CCL5 (RANTES)
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.

Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.

Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application   Neut - Quality tested

Recommended Usage
Each lot of this antibody is quality control tested by neutralization of the chemotaxis induced by recombinant mouse CCL5 (RANTES) (Cat. No. 594204) at 3 ng/mL on mouse Baf3-hCCR5 transfectant chemoattractant cells. ND50 range: 0.02 - 0.1 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details
Structure   Chemokine
Distribution
CCL5 is secreted by lymphocytes, macrophages, eosinophils, platelets, and fibroblasts. CCL5 is expressed by hepatocytes, sinusoidal endothelial cells and biliary epithelium during chronic hepatitis C.

Function
CCL5 can promote IL-2 and IL-5 expression in human T cells, and the expression of IL-6 and IL-8 in fibroblasts from patients with RA. CCL5 modulates LPS-induced IL-6 and TNFα in peripheral blood monocytes; this modulation occurs through the induction of IL-10 by CCL5.
Interaction
CCL5 is a chemoattractant for memory and activated T cells, monocytes, dendritic cells, eosinophils, NK cells, mast cells, and basophils.

Ligand/Receptor
CCL5 binds to CCR1, CCR3, and CCR5 receptors. CCL5 also binds to CCR9 but does not induce calcium flux, and therefore there is no chemotactic response.

Cell Type
B cells, Dendritic cells, Granulocytes, Macrophages, Monocytes
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family   Cytokines/Chemokines

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