产品说明: Clone SA271G2 (See other available formats) Regulatory Status RUO Other Names Ms4a1, Ly-44 IsotypeRat IgG2b, κ
描述 CD20是一种33-37kD的蛋白质,是MS4A家族的一员,具有四个跨膜区,当与MHC I类和II类、CD53、CD81和CD82结合时,这些跨膜区在细胞表面以同源寡聚复合物的形式存在。CD20在B细胞和T细胞亚群上表达,但在浆细胞上不表达。CD20调节B细胞的活化和增殖。其连接促进跨膜Ca2+运输。CD20是治疗B细胞淋巴瘤和白血病的重要治疗靶点。 CD20 is a 33-37 kD protein, a member of the MS4A family, with four transmembrane spanning regions that present as a homo-oligomeric complexes in the cell surface when associating with MHC class I and II, CD53, CD81, and CD82. CD20 is expressed on B cells and a subset of T cells, but not on plasma cells. CD20 regulates B-cell activation and proliferation. Its ligation promotes transmembrane Ca2+ trafficking. CD20 is an important therapeutic target in the treatment of B cell lymphomas and leukemias.
Product Details Isotype Control Ultra-LEAF™ Purified Rat IgG2b, κ Isotype Ctrl Verified Reactivity Mouse Antibody Type Monoclonal Host Species Rat Immunogen Mouse CD20 transfected cells
Formulation 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography. Concentration The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
Storage & Handling The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions. Application FC - Quality tested Depletion - Verified
Recommended Usage Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Clone SA271G2 has been validated for in vivo depletion of mouse B cells. Additional Product Notes View more applications data for this product in our Scientific Poster Library.
Watch a Scientific Poster video of this product.
Antigen Details Structure Member of the MS4A family, four transmembrane spanning regions, three isoforms of 37, 35, and 37 kD, depending on the phosphorylation degree. Distribution B cells and a subset of T cells
Function Regulates B-cell activation and proliferation Interaction Associates with MHC class I and II, CD53, CD81, and CD82 Cell Type B cells, T cells Biology Area Costimulatory Molecules, Immunology Molecular Family CD Molecules
以下是BioLegend 品牌部分产品,如需其他产品请联系本公司销售。 BioLegend 128307 PE anti-mouse DLL1 HMD1-3 50 ug BioLegend 128601 Purified anti-mouse CD34 HM34 50 ug BioLegend 128608 PerCP/Cyanine5.5 anti-mouse CD34 HM34 100 ug BioLegend 128609 PE anti-mouse CD34 HM34 50 ug BioLegend 128610 PE anti-mouse CD34 HM34 200 ug BioLegend 128611 APC anti-mouse CD34 HM34 25 ug BioLegend 128612 APC anti-mouse CD34 HM34 100 ug BioLegend 128618 PE/Cyanine7 anti-mouse CD34 HM34 100 ug BioLegend 128621 APC/Cyanine7 anti-mouse CD34 HM34 25 ug BioLegend 128622 APC/Cyanine7 anti-mouse CD34 HM34 100 ug BioLegend 128710 PE anti-mouse CD199 (CCR9) CW-1.2 100 ug BioLegend 128712 PE/Cyanine7 anti-mouse CD199 (CCR9) CW-1.2 100 ug BioLegend 128805 PE anti-mouse CD226 (DNAM-1) 1000000 25 ug BioLegend 128810 APC anti-mouse CD226 (DNAM-1) 1000000 100 ug BioLegend 128812 PE/Cyanine7 anti-mouse CD226 (DNAM-1) 1000000 100 ug BioLegend 128813 PerCP/Cyanine5.5 anti-mouse CD226 (DNAM-1) 1000000 25 ug BioLegend 128814 PerCP/Cyanine5.5 anti-mouse CD226 (DNAM-1) 1000000 100 ug BioLegend 128822 Purified anti-mouse CD226 (DNAM-1) 1000000 500 ug BioLegend 129602 Purified anti-mouse Siglec H 551 500 ug BioLegend 129605 PE anti-mouse Siglec H 551 25 ug BioLegend 129606 PE anti-mouse Siglec H 551 100 ug BioLegend 129608 Alexa Fluor® 647 anti-mouse Siglec H 551 100 ug