产品说明: Clone 1B7 (See other available formats) Regulatory Status RUO Other Names RNA binding protein fox-1 homolog 3, fox-1 homolog C, neuronal nuclei, hexaribonucleotide binding protein 3 Previously Covance Catalog# SIG-39860 Isotype Mouse IgG2b, κ
描述 Fox3是一个由3个哺乳动物Fox同源物组成的家族之一。Fox是在秀丽隐杆线虫中发现的一种与性别决定有关的基因,Fox这个名字是“X上的女性化位点”的缩写。Fox蛋白及其3种哺乳动物同源物均为约46kD的蛋白,每种蛋白都包括一个高度保守的RRM型RNA识别基序,该基序对应于一个由4条β链和两条α螺旋组成的约70个氨基酸的小结构。Fox3是一种具有RNA剪接功能的蛋白质,在神经元细胞核中大量特异性表达。1B7抗体显示Fox3/NeuN的强细胞核和远端细胞质染色,星形胶质细胞和其他类型的非神经元细胞完全没有染色。因此,这种Fox3/NeuN抗体是神经元细胞的优秀标志物。 Fox3 is one of a family of 3 mammalian Fox homologues. Fox was discovered in C. elegans as a gene involved in sex determination, and the name Fox is an acronym of "Feminizing locus on X". The Fox protein and its 3 mammalian homologues are all about 46 kD proteins, each of which includes a central highly conserved RRM type RNA recognition motif, which corresponds to a small ~70 amino acid structure consisting of 4 beta strands and two alpha-helices. Fox3 is a protein which has a function in RNA splicing and is expressed heavily and specifically in neuronal nuclei. The 1B7 antibody reveals strong nuclear and distal cytoplasmic staining for Fox3/NeuN and the complete absence of staining of astrocytes and other kinds of non-neuronal cells. This Fox3/NeuN antibody is therefore an excellent marker of neuronal cells.
Product Details Isotype Control Purified Mouse IgG2b, κ Isotype Ctrl Verified Reactivity Human, Mouse, Rat Antibody Type Monoclonal Host Species Mouse Immunogen Antibody was raised against the N-terminal 100 amino acids of human Fox3 as expressed in and purified from E. coli.
Formulation Phosphate-buffered solution + 10mM sodium azide. Preparation The antibody was purified by affinity chromatography. Concentration 1 mg/mL
Storage & Handling The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody. Application IHC-P - Quality tested WB, ICFC - Verified ICC, IHC-F - Reported in the literature, not verified in house
Recommended Usage Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.5 - 1.0 µg/mL is suggested. For western blotting, the suggested use of this reagent is 0.01 - 0.05 µg/mL. For intracellular flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Antigen Details Structure Expected MW: 46 kD and 48 kD Cell Type Mature Neurons, Neural Stem Cells Biology Area Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells Molecular Family Nuclear Markers
以下是 BioLegend 品牌部分产品,如需其他产品请联系本公司销售。 BioLegend 102201 Purified anti-mouse/rat CD29 HMβ1-1 50 ug BioLegend 102202 Purified anti-mouse/rat CD29 HMβ1-1 500 ug BioLegend 102205 FITC anti-mouse/rat CD29 HMβ1-1 50 ug BioLegend 102206 FITC anti-mouse/rat CD29 HMβ1-1 500 ug BioLegend 102207 PE anti-mouse/rat CD29 HMβ1-1 50 ug BioLegend 102208 PE anti-mouse/rat CD29 HMβ1-1 200 ug BioLegend 102211 Alexa Fluor® 488 anti-mouse/rat CD29 HMβ1-1 25 ug BioLegend 102216 APC anti-mouse/rat CD29 HMβ1-1 100 ug BioLegend 102221 PE/Cyanine7 anti-mouse/rat CD29 HMβ1-1 25 ug BioLegend 102225 APC/Cyanine7 anti-mouse/rat CD29 HMβ1-1 25 ug BioLegend 102226 APC/Cyanine7 anti-mouse/rat CD29 HMβ1-1 100 ug BioLegend 102227 PerCP/Cyanine5.5 anti-mouse/rat CD29 HMβ1-1 25 ug BioLegend 102228 PerCP/Cyanine5.5 anti-mouse/rat CD29 HMβ1-1 100 ug BioLegend 102233 TotalSeq™-A0570 anti-mouse/rat CD29 HMβ1-1 10 ug BioLegend 102306 PE anti-mouse CD30 mCD30.1 200 ug BioLegend 102401 Purified anti-mouse CD31 390 50 ug BioLegend 102405 FITC anti-mouse CD31 390 50 ug BioLegend 102406 FITC anti-mouse CD31 390 500 ug BioLegend 102408 PE anti-mouse CD31 390 200 ug BioLegend 102409 APC anti-mouse CD31 390 25 ug BioLegend 102410 APC anti-mouse CD31 390 100 ug BioLegend 102413 Alexa Fluor® 488 anti-mouse CD31 390 25 ug