产品说明: Clone 7A6 (See other available formats) Regulatory Status RUO Other Names Nuclear factor of activated T-cells, cytoplasmic 1, NFAT transcription complex cytosolic component, NFAT2, NFATc Isotype Mouse IgG1, κ
描述 该基因的产物是活化的T细胞DNA结合转录复合物的核因子的组成部分。蛋白质复合物由NFAT1、NFAT2(NFATc1或NFATc)、NFAT3和NFAT4组成。该家族的所有成员都是具有Rel同源结构域的转录因子,并与AP-1(Jun/Fos)协同调节基因转录以协调有效的免疫反应。NFAT蛋白主要在免疫系统的细胞中表达,但也在骨骼肌、角质形成细胞和脂肪细胞中表达、调节这些细胞中的细胞分化程序。在静息细胞中,NFAT蛋白被高度磷酸化并定位在细胞质中。细胞内钙浓度的增加激活了钙/钙调素依赖性丝氨酸磷酸酶钙调磷酸酶,使NFAT蛋白脱磷,导致其随后易位到细胞核。 属于该转录因子家族的蛋白质在免疫应答过程中的诱导型基因转录中起着核心作用。该基因的产物是一种可诱导的核成分。它是免疫抑制药物如环孢菌素a的主要分子靶点。已经为该基因鉴定了五种编码不同异构体的转录物变体。该蛋白的不同亚型可能调节不同细胞因子基因的诱导表达。 The product of this gene is a component of the nuclear factor of activated T cells DNA-binding transcription complex. The protein complex consists of NFAT1, NFAT2 (NFATc1 or NFATc), NFAT3, and NFAT4. All members of this family are transcription factors with a Rel homology domain and regulate gene transcription in concert with AP-1 (Jun/Fos) to orchestrate an effective immune response. NFAT proteins are predominantly expressed in cells of the immune system but are also expressed in skeletal muscle, keratinocytes and adipocytes, regulating cell differentiation programs in these cells. In resting cells, NFAT proteins are heavily phosphorylated and localized in the cytoplasm. Increased intracellular calcium concentrations activate the calcium/calmodulin-dependent serine phosphatase calcineurin, which dephosphorylates NFAT proteins, resulting in their subsequent translocation to the nucleus.
Proteins belonging to this family of transcription factors play a central role in inducible gene transcription during immune response. The product of this gene is an inducible nuclear component. It functions as a major molecular target for the immunosuppressive drugs such as cyclosporin A. Five transcript variants encoding distinct isoforms have been identified for this gene. Different isoforms of this protein may regulate inducible expression of different cytokine genes.
Product Details Isotype Control Purified Mouse IgG1, κ Isotype Ctrl Verified Reactivity Human, Mouse, Rat Antibody Type Monoclonal Host Species Mouse Immunogen Recombinant protein of human NFATc1 amino acids 197-304.
Formulation This antibody is provided in phosphate-buffered solution, pH 7.2, 0.09% sodium azide.
Preparation The antibody was purified by affinity chromatography. Concentration 0.5 mg/mL Storage & Handling Upon receipt, store undiluted between 2°C and 8°C.
Application WB - Quality tested ChIP - Verified FC - Reported in the literature, not verified in house ICC - Reported by the developer, not verified in house
Recommended Usage Each lot of this antibody is quality control tested by Western blotting. For Western blotting applications, a concentration of 1 µg/mL is recommended. For ChIP applications, the suggested dilution is is 1:100-1:2000 by volume. It is recommended that the reagent be titrated for optimal performance for each application.
Antigen Details Structure 943 amino acids with predicted molecular weight of 101kD. There are 10 isoforms with a predicted MW of 39kD, 74kD, 76kD, 77kD (3), 88kD (2), 100kD, 101kD.
Distribution Cytoplasm, Nucleus. NFATc1 is cytoplasmic in the phosphorylated form and nuclear after calcineurin-mediated dephosphorylation controlled activation.
Biology Area Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Transcription Factors Molecular Family Nuclear Markers