BioLegend 753406 Recombinant H

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BioLegend 753406 Recombinant Human S100A8/A9 Heterodimer (carrier-free)
产品简介：
产品品牌：BioLegend
产品货号：753406
产品名称：BioLegend 753406 Recombinant Human S100A8/A9 Heterodimer (carrier-free)
产品规格：100 µg

产品说明：
Regulatory Status   RUO
Other Names
Calprotectin, Human S100A8 (Myeloid-related protein, MRP 8), S100A9 (MRP14), calgranulin A and B, L1, cystic fibrosis antigen, and 27E10 antigen, P8, MIF, NIF, CAGA, CFAG, CGLA, L1Ag, MRP8, CP-10, MA387, 60B8AG

描述
S100A8/A9，也称为钙卫蛋白，是一种异二聚体蛋白，属于具有两个EF手钙结合结构域的低分子量钙结合S100蛋白家族。钙和锌的结合影响S100A8/A9的构象和低聚状态，包括自组装成同源和异二聚体以及较大的低聚物。S100A8/A9在许多病理状况下在血液和其他体液中升高，包括类风湿性关节炎、炎症性肠病、病毒或微生物感染以及肿瘤。S100A8/A9被鉴定为TLR4的内源性激活剂，并参与促进感染中的炎症反应。中性粒细胞衍生的S100A8/A9通过螯合锰和锌来抑制金黄色putao球菌的生长。S100A8/A9在自身免疫以及癌症发展和肿瘤扩散中也被鉴定为炎症的有效放大器。S100A8/A9诱导的损伤刺激单核细胞和巨噬细胞产生IP-10。此外，S100A8/A9异二聚体还促进星形胶质细胞增殖。
S100A8/A9, also known as Calprotectin, is a heterodimeric protein that belongs to the family of low molecular weight, calcium binding S100 proteins with two EF-hand calcium binding domains. Calcium and zinc binding influences the S100A8/A9 conformation and oligomerization state, which includes the self-assembly into homo- and heterodimers and larger oligomers. S100A8/A9 is elevated in blood and other body fluids in many pathological conditions including rheumatoid arthritis, inflammatory bowel diseases, viral or microbial infections, and tumors. S100A8/A9 is identified as an endogenous activator of TLR4 and is involved in promoting the inflammatory response in infections. Neutrophil-derived S100A8/A9 inhibits growth of Staphylococcus aureus through the chelation of manganese and zinc. S100A8/A9 is also identified as a potent amplifier of inflammation in autoimmunity as well as in cancer development and tumor spread. Injury induced by S100A8/A9 stimulates IP-10 production in monocytes and macrophages. In addition, the S100A8/A9 heterodimer also promotes astrocyte proliferation.

Product Details
Source
Human S100A8, amino acids (Met1-Glu93) (Accession# P05109), and S100A9, amino acids (Thr2-Pro114) (Accession# P06702), were expressed in E. coli.

Molecular Mass
The 206 amino acid recombinant heterodimeric protein has a predicted molecular mass of approximately 23.9 kD (~11 kD for S100A8 and ~13 kD for S100A9). Under reducing conditions, the proteins migrate approximately at 9 kD (S100A8) and 13 kD (S100A9) by SDS-PAGE. The predicted N-terminal amino acids are Met (S100A8) and Thr (S100A9) for the corresponding monomers.

Purity
>95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in 2 mM DTT, PBS, pH 7.2.
Endotoxin Level
Less than 0.01 ng per µg cytokine as determined by the LAL method.

Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.

Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.

Activity
The ED50 is 0.15 - 0.6 µg/mL as measured by its ability to induce proliferation of astrocytes.
Application   Bioassay

Antigen Details
Structure   Non-covalent heterodimer.
Distribution
Neutrophils, monocytes, macrophages, gingival keratinocytes, granulocytes, osteoclasts, fibroblasts, and microvascular endothelial cells.

Function
Pro-inflammatory molecule that possesses antimicrobial properties. Is up-regulated in neutrophils and monocytes at the sites of inflammation.

Interaction   Astrocytes, neutrophils, and monocytes.
Ligand/Receptor   RAGE and TLR4.
Bioactivity
S100A8/A9 Heterodimer induces the proliferation of astrocytes.

Biology Area
Cell Biology, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family    Cytokines/Chemokines, Growth Factors

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