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- 文献和实验
- 技术资料
- 库存：
99
- 英文名：
Recombinant Mouse TNF-α (Animal-Free)
- 供应商：
北京云肽生物科技有限公司
BioLegend 718004 Recombinant Mouse TNF-α (Animal-Free)
北京云肽生物科技有限公司作为 BioLegend 的特约经销商，为广大客户提供原装正品，货期短，折扣好的服务准则。
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BioLegend 718004 Recombinant Mouse TNF-α (Animal-Free)
产品简介：
产品品牌：BioLegend
产品货号：718004
产品名称：BioLegend 718004 Recombinant Mouse TNF-α (Animal-Free)
产品规格：20 µg
产品说明：
Regulatory Status RUO
Other Names
Tumor necrosis factor-α, Cachectin, Necrosin, Macrophage cytotoxic factor (MCF), Differentiation inducing factor (DIF), TNFSF2
描述
TNF-α由巨噬细胞、单核细胞、中性粒细胞、T细胞（主要是CD4+）和NK细胞分泌。许多转化的细胞系也分泌TNF-α。TNF-α形成多聚体复合物；稳定的三聚体在溶液中最常见。还描述了一种26 kD膜形式的TNF-α。TNF-α与表面受体的结合引发了广泛的生物活性，包括：体外许多肿瘤细胞系的细胞溶解和细胞停滞，体内肿瘤的出血性坏死，增加成纤维细胞增殖，增强中性粒细胞的趋化性和吞噬作用。
TNF-α is secreted by macrophages, monocytes, neutrophils, T-cells (principally CD4+), and NK-cells. Many transformed cell lines also secrete TNF-α. TNF-α forms multimeric complexes; stable trimers are most common in solution. A 26 kD membrane form of TNF-α has also been described. TNF-α binding to surface receptors elicits a wide array of biologic activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorraghic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.
Product Details
Source
Mouse TNF-α, amino acids Leu80-Leu235 (Accession# NM_013693), was expressed in E. coli.
Molecular Mass
The 157 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 17.4 kD. The predicted N-terminal amino acid is Met.
Purity
>98%, as determined by Coomassie stained SDS-PAGE and HPLC analysis.
Formulation Lyophilized, carrier-free.
Endotoxin Level Less than 0.1 ng per µg of protein.
Storage & Handling
Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. It is recommended to further dilute in a buffer, such as 5% Trehalose, and store working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
Activity
The expected ED50 is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1.0 x 107 units/mg, as determined by the cytolysis of murine L929 cells in the presence of Actinomyocin D.
Application Bioassay
Antigen Details
Structure Cytokine
Distribution
Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells
Function
Type II integral membrane protein processed by TACE for secretion; upregulated by interferons, IL-2, GM-CSF, substance P, bradykinin, PAF, immune complexes, cyclooxygenase; downregulated by IL-6, TGF-β, vitamin D3, prostaglandin E2, PAF antagonists
Ligand/Receptor
TNFRSF1A (TNF-R1, CD120a, TNFR-p60 Type β, p55), TNFRSF1B (TNF-R2, CD120b, TNFR-p80 Type A, p75)
Bioactivity
Paracrine/endocrine mediator of inflammatory and immune functions; selectively cytotoxic for transformed cells; endothelial cell alterations; chemoattractant
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family Cytokines/Chemokines
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BioLegend 718004 Recombinant Mouse TNF-α (Animal-Free)
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