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- 详细信息
- 文献和实验
- 技术资料
- 库存：
99
- 英文名：
Recombinant Human TNF-α (Animal-Free)
- 供应商：
北京云肽生物科技有限公司
BioLegend 717904 Recombinant Human TNF-α (Animal-Free)
北京云肽生物科技有限公司作为 BioLegend 的特约经销商，为广大客户提供原装正品，货期短，折扣好的服务准则。
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BioLegend 717904 Recombinant Human TNF-α (Animal-Free)
产品简介：
产品品牌：BioLegend
产品货号：717904
产品名称：BioLegend 717904 Recombinant Human TNF-α (Animal-Free)
产品规格： 50 µg
产品说明：
Regulatory Status RUO
Other Names
Tumor necrosis factor-α, Cachectin, Necrosin, Macrophage cytotoxic factor (MCF), Differentiation inducing factor (DIF), TNFSF2
描述
TNF-α从巨噬细胞、单核细胞、中性粒细胞、T细胞（主要是CD4+）、NK细胞和许多转化的细胞系中释放。可溶性同源三聚体TNF-α通过TNF-α的整体膜前体形式的蛋白水解从细胞中释放。TNF-α与一些TNF-α受体结合可诱导细胞凋亡，并且根据细胞类型、受体表达和信号转导状态，可诱导其他反应。TNF-α参与炎症反应。
TNF-α is released from macrophages, monocytes, neutrophils, T-cells (principally CD4+), NK-cells and many transformed cell lines. Soluble homotrimeric TNF-α is released from cells by proteolysis of the integral membrane precursor form of TNF-α. TNF-α binding to some TNF-α receptors induces apoptosis and, depending on cell type, receptor expression, and signal transduction status, can induce other responses. TNF-α is involved in the inflammatory response.
Product Details
Source
Human TNF-α, amino acids Val77-Leu233 (Accession# NM_000594), was expressed in E. coli.
Molecular Mass
The 157 amino acid recombinant protein has a predicted molecular mass of 17.4 kD. The predicted N-terminal amino acid is Val.
Purity
>98%, as determined by Coomassie stained SDS-PAGE and HPLC analysis.
Formulation Lyophilized, carrier-free.
Endotoxin Level Less than 0.1 ng per µg of protein.
Storage & Handling
Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. It is recommended to further dilute in a buffer, such as 5% Trehalose, and store working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
Activity
The expected ED50 is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2.0 x 107 units/mg, as determined by the cytolysis of murine L929 cells in the presence of Actinomyocin D.
Application Bioassay
Application Notes
This product is reactive with human, cow, monkey, mouse, rat, sheep, dog, and pig.
Antigen Details
Structure Cytokine
Distribution
Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells.
Function
Type II integral membrane protein processed by TACE for secretion; upregulated by interferons, IL-2, GM-CSF, substance P, bradykinin, PAF, immune complexes, cyclooxygenase; downregulated by IL-6, TGF-β, vitamin D3, prostaglandin E2, PAF antagonists.
Interaction
Monocytes, neutrophils, macrophages, T cells, fibroblasts, endothelial cells, osteoclasts, adipocytes, astroglia, microglia.
Ligand/Receptor
TNFRSF1A (TNF-R1, CD120a, TNFR-p60 Type β, p55); TNFRSF1B (TNF-R2, CD120b, TNFR-p80 Type A, p75)
Bioactivity
Paracrine/endocrine mediator of inflammatory and immune functions; selectively cytotoxic for transformed cells; endothelial cell alterations; chemoattractant.
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family Cytokines/Chemokines
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文献和实验相关实验
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BioLegend 717904 Recombinant Human TNF-α (Animal-Free)
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