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- 库存：
99
- 英文名：
Recombinant Human LIF (Animal-Free)
- 供应商：
北京云肽生物科技有限公司
BioLegend 716404 Recombinant Human LIF (Animal-Free)
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BioLegend 716404 Recombinant Human LIF (Animal-Free)
产品简介：
产品品牌：BioLegend
产品货号：716404
产品名称：BioLegend 716404 Recombinant Human LIF (Animal-Free)
产品规格：25 µg
产品说明：
Regulatory Status RUO
Other Names
Leukemia inhibitory factor, HILDA (human interleukin in DA cells), MLPLI (melanoma-derived LPL inhibitor), DIA (differentiation-inducing factor)
描述
白血病抑制因子（LIF）是一种20 kD的蛋白，属于IL-6受体家族。它与由LIF特异性亚基gp190或LIFR和与IL-6家族其他成员共享的亚基gp130组成的异二聚体膜受体结合。LIF复合物受体通过STAT3和JAK激酶发出信号。在包括胸腺、肺和神经元组织在内的各种组织中观察到LIF的表达。LIF表现出多种生物学效应，但最为人所知的是其抑制小鼠胚胎干细胞分化和促进干细胞自我更新的能力。人和小鼠LIF具有79%的序列同源性，并表现出跨物种活性。然而，LIF对干细胞分化的抑制似乎是小鼠特异性的。它参与诱导正常和髓系白血病细胞的造血分化、诱导神经元细胞分化、调节肾脏发育过程中的间充质向上皮转化，还可能在母婴界面的免疫耐受中发挥作用。对于该基因，已经观察到编码多种异构体的选择性剪接转录物变体。LIF可被促炎细胞因子如TNF-α和IL-17上调，在类风湿性关节炎、神经损伤、全身炎症和结核病的病例中发现LIF水平升高。
Leukemia inhibitory factor (LIF) is a 20 kD protein belonging to the IL-6 receptor family. It binds to a heterodimer membrane receptor composed of LIF specific subunit gp190 or LIFR and subunit gp130 shared with other members of the IL-6 family. The LIF complex receptor emits signals through STAT3 and JAK kinases. LIF expression was observed in various tissues, including thymus, lung, and neuronal tissue. LIF exhibits various biological effects, but the most well-known one is its ability to inhibit the differentiation of mouse embryonic stem cells and promote stem cell self-renewal. Human and mouse LIFs have 79% sequence homology and exhibit cross species activity. However, the inhibition of stem cell differentiation by LIF seems to be mouse specific. It is involved in inducing hematopoietic differentiation of normal and myeloid leukemia cells, inducing neuronal cell differentiation, regulating mesenchymal to epithelial transformation during renal development, and may also play a role in immune tolerance at the mother infant interface. Selective splicing transcript variants encoding multiple isomers have been observed for this gene. LIF can be stimulated by pro-inflammatory cytokines such as TNF- α And IL-17 were up-regulated, and LIF levels were found to be elevated in patients with rheumatoid arthritis, nerve injury, systemic inflammation and tuberculosis.
Product Details
Source
Human LIF, amino acids (Ser23-Phe202) (Accession# NP_002300.1), was expressed in E. coli.
Molecular Mass
The 180 amino acid recombinant protein has a predicted molecular mass of 19.6 kD. The predicted N-terminal amino acid is Ser.
Purity
>98%, as determined by Coomassie stained SDS-PAGE and HPLC analysis.
Formulation Lyophilized, carrier-free.
Endotoxin Level Less than 0.1 ng per µg of protein.
Storage & Handling
Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. It is recommended to further dilute in a buffer, such as 5% Trehalose, and store working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
Activity
ED50 is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1.0 x 107 units/mg as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells.
Application Bioassay
Antigen Details
Structure Cytokine
Distribution
Expressed in the trophectoderm of the developing embryo, activated CD4 T cells. +
Function
Inhibits embryonic stem cell differentiation, induces the terminal differentiation of myeloid leukemia cells. LIF is upregulated by TNF-α and IL-17. LIF is downregulated by IL-4 and IL-13 in fibroblasts.
Interaction Embryonic stem cells.
Ligand/Receptor LIFR complex (gp190 and gp130).
Bioactivity Human LIF induces proliferation of TF-1 cells.
Cell Type
Hematopoietic stem and progenitors, Embryonic Stem Cells
Biology Area Cell Biology, Immunology, Stem Cells
Molecular Family Growth Factors, Cytokines/Chemokines
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BioLegend 716404 Recombinant Human LIF (Animal-Free)
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