BioLegend 689202 HRP Protein A

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BioLegend 689202 HRP Protein A
产品简介：
产品品牌：BioLegend
产品货号：689202
产品名称：BioLegend 689202 HRP Protein A
产品货号： 500 µL

产品说明：
Regulatory Status   RUO
Other Names   Protein A

描述
蛋白A是一种42kD的表面蛋白，最初存在于金黄色putao球菌的细胞壁中。由于其结合免疫球蛋白的能力，它被广泛应用于生物化学研究和工业中。它由五个同源的Ig结合结构域组成，这些结构域折叠成三螺旋束。每个结构域都能够结合许多哺乳动物物种的蛋白质，尤其是IgG。它结合大多数免疫球蛋白Fc区内的重链。HRP蛋白A可用于检测初级抗体，例如在ELISA、ELISPOT、IHC或蛋白质印迹中。对于免疫印迹后的免疫印迹应用，HRP蛋白A将避免IgG重链/轻链问题，因为它只识别天然形式的IgG，并且用于免疫印迹靶标的抗体通常与免疫沉淀物一起煮沸并变性。
Protein A is a 42 kD surface protein originally found in the cell wall of the bacterium 金黄色葡萄糖jun. It is widely used in biochemical research and industry because of its ability to bind immunoglobulins. It is composed of five homologous Ig-binding domains that fold into a three-helix bundle. Each domain is able to bind proteins from many mammalian species, most notably IgGs. It binds the heavy chain within the Fc region of most immunoglobulins. HRP Protein A is useful for detecting primary antibodies, e.g., in ELISA, ELISPOT, IHC, or Western blotting. For IP followed by Western blotting application, HRP protein A will avoid IgG heavy/light chain issue because it only recognizes the IgG in its native form, and the antibody used to IP the target is usually boiled with the immunoprecipitates and gets denatured.

Product Details
Verified Reactivity   Mouse, Rabbit
Formulation
This reagent is provided in 50% glycerol in aqueous buffered solutions with preservatives.

Preparation
Recombinant protein A was conjugated with HRP under optimal conditions.
Concentration   1.0 mg/ml

Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application   WB - Quality tested

Recommended Usage
For Western blot analysis, the reagent should be titrated between 1:2000 - 1:8000 to determine optimal conditions.

Antigen Details
Structure   42 kD surface protein.
Function   Binds IgG.
Biology Area   Cell Biology

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