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- BioLegend
- 2025年11月15日
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- 技术资料
- 库存：
99
- 英文名：
Direct-Blot™ HRP anti-HA.11 Epitope Tag
- 供应商：
北京云肽生物科技有限公司
北京云肽生物科技有限公司作为 BioLegend 的特约经销商，为广大客户提供原装正品，货期短，折扣好的服务准则。
美国 BioLegend 公司位于美国加州，致力于为生物医药研究的各个前沿领域提供高质量的抗体及其它相关试剂。BioLegend 虽然成立的时间不长，但它聚集了一批来自 PharMingen 公司的专业人士，建立了一支具有雄厚技术背景的专业队伍。通过其自身的先天优势，以及与世界知名实验室和研究所的良好合作关系，以合理的价格为广大客户提供高质量的免疫学和细胞生物学产品。
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BioLegend 901519 Direct-Blot™ HRP anti-HA.11 Epitope Tag
产品简介：
产品品牌：BioLegend
产品货号：901519
产品名称：BioLegend 901519 Direct-Blot™ HRP anti-HA.11 Epitope Tag
产品规格：100 µL
产品说明：
Clone 16B12 (See other available formats)
Regulatory Status RUO
Other Names
HA epitope tag, HA1, HA2, hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain, YPYDVPDYA, Hemagglutinin tag
Isotype Mouse IgG1, κ
描述
HA标签（血凝素）是衍生自人流感血凝素表面糖蛋白的氨基酸序列，对应于氨基酸98-106。它通常被用作标签，以促进蛋白质的检测、分离和纯化。完整的氨基酸序列为：YPYDVPDYA。
The HA tag (hemagglutinin) is an amino acid sequence derived from the human influenza hemagglutinin surface glycoprotein, corresponding to amino acids 98-106. It is commonly used as a tag to facilitate detection, isolation, and purification of proteins. The full amino acid sequence is: YPYDVPDYA.
Product Details
Antibody Type Monoclonal
Host Species Mouse
Immunogen
Monoclonal antibody HA.11 was raised against the twelve amino acid peptide CYPYDVPDYASL.
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application WB - Quality tested
Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:10000. The optimal dilution should be determined by titration for each individual assay of interest.
25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.
Antigen Details
Biology Area Cell Biology+
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文献和实验Studies of the Ubiquitin Proteasome System
from other investigators) Transfection efficiency control plasmid, e.g., GFP, RFP, β‐galactosidase 2× HEPES‐buffered saline (HeBS; see recipe ) Anti– tag epitope antibody or anti‐substrate
v468.Chapter 3 分泌型跨膜相关蛋白提纯及Wnt抑制活性
primary antibody solution, and wash membrane 4×4 min with TTBS at room temperature. 11. After the last wash, incubate the membrane in solution containingHRP–secondary antibody conjugate (donkey anti-goatIgG-HRP at a 1:1,000 dilution) for 1 h at room
Identification and Analysis of Multiprotein Complexes Through Chemical Crosslinking
was treated with TEV protease prior to denaturation and immunoblotting with anti‐HA (heme agglutinin epitope tag) antibody. The cleavage site for TEV protease is indicated by a thick bar. Modified from Klein et al., ; see Klein et al., for details
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