BioLegend 656108 Alexa Fluor®

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BioLegend 656108 Alexa Fluor® 647 anti-SOX2
产品简介：
产品品牌：BioLegend
产品货号：656108
产品名称：BioLegend 656108 Alexa Fluor® 647 anti-SOX2
产品规格：100 µg

产品说明：
Clone   14A6A34 (See other available formats)
Regulatory Status   RUO
Other Names
SRY-related HMG-box gene 2, SRY (sex determining region Y)-box 2, MCOPS3, ANOP3
Isotype   Mouse IgG1, κ

描述
SOX2是SRY相关盒转录因子家族中研究最多的成员。它通过其高度保守的HMG-box结构域与靶基因结合。SOX2基因的失活在胚胎发育过程中会导致死亡。胚胎干细胞中SOX2的敲除导致其分化。SOX与OCT4、MYC和KLF4的共表达足以将体细胞重新编程为诱导多能干细胞（iPSC），其发挥与天然多能干细胞相似的特性。这些发现表明SOX2对胚胎干细胞的自我更新和多能性至关重要。此外，在各种类型的恶性癌症中发现了SOX2的过度表达。敲除SOX2通过下调细胞周期蛋白D1和抑制肿瘤细胞增殖导致细胞周期停滞，表明SOX2参与激活与肿瘤进展相关的基因。
SOX2 is the most studied member of SRY-related box transcription factor family. It binds to target genes through its highly conserved HMG box domain. Inactivation of the SOX2 gene causes lethality during embryonic development. SOX2 knockdown in embryonic stem cells results in their differentiation. Co-expression of SOX with OCT4, MYC, and KLF4 is sufficient to reprogram somatic cells to induced pluripotent stem cells (iPSCs), which exert similar characteristics as natural pluripotent stem cells. These findings indicate that SOX2 is crucial for the self-renewal and pluripotency of embryonic stem cells. In addition, over-expression of SOX2 has been found in various types of malignant cancer. Knockdown of SOX2 results in cell cycle arrest by downregulating cyclin D1 and inhibition of tumor cell proliferation, suggesting that SOX2 is involved in activating genes associated with tumor progression.

Product Details
Isotype Control
Alexa Fluor® 647 Mouse IgG1, κ Isotype Ctrl (ICFC)
Verified Reactivity   Human, Mouse
Antibody Type   Monoclonal
Host Species   Mouse
Immunogen
Full length human SOX recombinant protein

Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration   0.5 mg/ml

Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application   ICC - Quality tested
FC - Verified

Recommended Usage
Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

Antigen Details
Structure
317 amino acids, predicted molecular weight of 34 kD, contains a HMG box domain responsible for DNA binding
Distribution   Nucleus

Function
Transcription factor that regulates the expression of the genes involved in embryonic development
Interaction    Interacts with FGFR1, SOX3, and ZSCAN10
Cell Type
Embryonic Stem Cells, Mesenchymal Stem Cells, Neural Stem Cells

Biology Area
Cell Biology, Cell Cycle/DNA Replication, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells, Transcription Factors

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