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- 库存：
99
- 英文名：
Recombinant Mouse Noggin (carrier-free)
- 供应商：
北京云肽生物科技有限公司
BioLegend 597104 Recombinant Mouse Noggin (carrier-free)
北京云肽生物科技有限公司作为 BioLegend 的特约经销商，为广大客户提供原装正品，货期短，折扣好的服务准则。
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BioLegend 597104 Recombinant Mouse Noggin (carrier-free)
产品简介：
产品品牌：BioLegend
产品货号：597104
产品名称：BioLegend 597104 Recombinant Mouse Noggin (carrier-free)
产品规格：20 µg
描述
诺金是一种胚胎诱导剂，可以以极高的亲和力螯合BMP家族的TGF-β细胞因子。该蛋白最初被鉴定为BMP-4拮抗剂，其作用对头部和其他背侧结构的正确形成至关重要。诺金还被证明可以调节其他BMP的活性，包括BMP-2、-7、-13和-14。它在成人中枢神经系统的特定区域和肺、骨骼肌和皮肤等外周组织中表达。在某些条件下培养人胚胎干细胞（hESC）或神经干细胞期间，添加Noggin以拮抗BMP活性可以使干细胞增殖，同时保持其未分化状态，或者分化为多巴胺能神经元。诺金似乎也能在体内维持成体干细胞群。例如，它将神经干细胞维持在海马体内。Noggin在小鼠中的靶向缺失导致产前死亡和显示严重畸形骨骼系统的隐性表型。相反，在成熟成骨细胞中过表达Noggin的转基因小鼠表现出成骨细胞分化受损、骨形成减少和严重骨质疏松。
Noggin is an embryonic inducer that can sequester TGF-β cytokines of the BMP family with extremely high affinity. This protein was originally identified as a BMP-4 antagonist whose action is critical for proper formation of the head and other dorsal structures. Noggin has also been shown to modulate the activities of other BMPs including BMP-2, -7, -13, and -14. It is expressed in defined areas of the adult central nervous system and peripheral tissues such as lung, skeletal muscle, and skin. During culture of human embryonic stem cells (hESC) or neural stem cells under certain conditions, addition of Noggin to antagonize BMP activity may allow stem cells to proliferate while maintaining their undifferentiated state, or alternatively, to differentiate into dopaminergic neurons. Noggin also appears to maintain adult stem cell populations in vivo. For example, it maintains neural stem cells within the hippocampus. Targeted deletion of Noggin in mice results in prenatal death and a recessive phenotype displaying a severely malformed skeletal system. Conversely, transgenic mice over-expressing Noggin in mature osteoblasts display impaired osteoblastic differentiation, reduced bone formation, and severe osteoporosis.
Product Details
Source
Mouse Noggin, amino acids Gln28-Cys232 (Accession# NP_032737.1), with an additional Met at the N-terminus, was expressed in E. coli.
Molecular Mass
Recombinant Mouse Noggin is a 46.4 kD disulfide-linked homodimer consisting of two 206 amino acid polypeptide chains. The predicted N-terminal amino acid is Met.
Purity
>95%, as determined by Coomassie stained SDS-PAGE.
Formulation Lyophilized
Endotoxin Level Less than 0.1 ng per µg of protein.
Storage & Handling
Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. Note: Due to solubility reasons the protein should be kept at low pH. It is recommended to further dilute in a buffer containing a carrier protein such as 0.1% BSA and store working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
Activity
Mouse Noggin is able to inhibit 5.0 ng/ml of BMP-4 induced alkaline phosphatase production by ATDC5 chondrogenic cells with an ED50 of 1.0 - 2.0 ng/ml, corresponding to a specific activity of 5.0 x 105 - 1.0 x 106 units/mg.
Application Bioassay
Antigen Details
Structure Cytokine
Distribution Skeletal muscle, lung, neuron, skin
Function Antagonizes BMPs.
Interaction Mesenchymal stem cells
Ligand/Receptor BMPs
Bioactivity
mNOG is able to inhibit BMP-4 induced alkaline phosphatase production by ATDC chondrogenic cells.
Cell Type Mesenchymal Stem Cells, Embryonic Stem Cells
Biology Area
Cell Biology, Immunology, Neuroscience, Signal Transduction, Stem Cells, Synaptic Biology
Molecular Family Growth Factors, Cytokines/Chemokines
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BioLegend 597104 Recombinant Mouse Noggin (carrier-free)
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