BioLegend 554306 Recombinant H

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BioLegend 554306 Recombinant Human MMP-2 (carrier-free)
产品简介：
产品品牌：BioLegend
产品货号：554306
产品名称：BioLegend 554306 Recombinant Human MMP-2 (carrier-free)
产品规格： 100 µg

产品说明：
Regulatory Status  RUO
Other Names
Matrix metallopeptidase 2, Gelatinase A, MMP2, GelA, Clg4a

描述
基质金属蛋白酶2，也称明胶酶A，是基质金属蛋白酶家族蛋白（MMPs）的成员。MMPs是结构相关的含锌酶，在胚胎发育、繁殖和组织重塑等正常生理过程以及关节炎和转移等疾病过程中降解细胞外基质和结缔组织蛋白。MMP-2由激活时被切割的前结构域、含有锌结合位点的催化结构域、纤连蛋白样结构域（在底物靶向中起作用）和羧基末端（血红素样重复序列）结构域组成。MMP-2的激活需要蛋白水解处理：首先，膜型MMP（MT1-MMP）和金属蛋白酶组织抑制剂2的复合物将pro-MMP-2从细胞外环境募集到细胞表面；其次，MMP-2被活性MT1-MMP和随后的自催化切割激活。MMP-2的底物包括IV型胶原、聚集蛋白聚糖、连接蛋白、去胶蛋白、纤连蛋白以及X型和XI型胶原，所有这些都是关节软骨基质的成分。重要的是，MMP-2分泌在几种类型的人类癌症中升高，其表达升高与预后不良有关。MMP-2基因突变与Torg-Winchester综合征、多中心骨溶解、关节炎综合征以及可能的瘢痕疙瘩有关。MMP-2缺陷小鼠表现出轻微的生长延迟、新生血管减少、肿瘤进展迟缓、对过敏原的哮喘反应过度以及乳腺分支形态发生受损。
MMP-2, also named gelatinase A, is a member of matrix metalloproteinase family proteins (MMPs). MMPs are structurally related, zinc-containing enzymes that degrade the extracellular matrix and connective tissue proteins in normal physiological processes such as embryonic development, reproduction, and tissue remodeling as well as in disease processes such as arthritis and metastasis. MMP-2 consists of a prodomain, which is cleaved upon activation, a catalytic domain containing the zinc binding site, a fibronectin-like domain (that plays a role in the substrate targeting), and a carboxyl-terminal (hemopexin-like repeats) domain. Activation of MMP-2 requires proteolytic processing: first, a complex of membrane type 1 MMP (MT1-MMP) and tissue inhibitor of metalloproteinase 2 recruits pro-MMP-2 from the extracellular milieu to the cell surface; second, MMP-2 is activated by active MT1-MMP and subsequent autocatalytic cleavage. Substrates of MMP-2 include type IV collagen, aggrecan, link protein, decorin, fibronectin, and type X and XI collagens, all of which are components of the articular cartilaginous matrix. Importantly, MMP-2 secretion is elevated in several types of human cancers and its elevated expression has been associated with a poor prognosis. Mutations in the MMP-2 gene are associated with Torg-Winchester syndrome, multicentric osteolysis, arthritis syndrome, and possibly keloids. MMP-2 deficient mice exhibit slightly delayed growth, reduced neovascularization, retarded tumor progression, an exaggerated asthma response to allergens, and impaired branching morphogenesis of the mammary gland.

Product Details
Source
Human MMP-2, amino acids (Ala30-Cys660) (Accession# NP_004521.1) with a C-terminal His tag and a linker sequence, was expressed in 293E cells.

Molecular Mass
This 652 amino acid recombinant protein has a predicted molecular mass of approximately 73.2 kD. The protein migrates at about 73 kD in DTT-reducing conditions and about 73 kD in non-reducing conditions by SDS-PAGE.The predicted N-terminal amino acid is Ala.

Purity
>95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in TCN (25 mM TRIS, 5 mM CaCl2, 150 mM NaCl, pH 7.5).

Endotoxin Level
Less than 1.0 EU per µg of protein as determine by the LAL method
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.

Storage & Handling
Unopened vial can be stored at -20°C or -70°C for six months. For maximum results, quick spin vial prior to opening. Avoid repeated freeze/thaw cycles.

Activity
Human MMP-2 cleaves the modified fluorogenic peptide Mca-PLGL-Dpa-AR-NH2 with an activity above 1100 pmol/min/µg.
Application   Bioassay

Application Notes
Human MMP-2 at a concentration 100 µg/mL was activated for at least 1 h at 37°C in the presence of 1 mM APMA to produce activated MMP-2. The final assay condition was 10 ng of activated MMP-2 and 10 µM of substrate. Reaction was read at excitation and emission of 320 nm and 405 nm, respectively, in kinetic mode for 5 min.
 
This protein is in the latent form and needs to be activated for bioassay.

Antigen Details
Structure   Monomer
Distribution
MMP-2 is expressed by fibroblasts, endothelial cells, alveolar epithelial cells, and macrophages.

Function
Degradation of ECM and connective tissue proteins, angiogenesis, tissue remodeling, cancer metastasis and MMP-2 secretion is elevated in several types of cancers.
Ligand/Receptor    TIMPs, CCL7, thrombospondin 1.
Bioactivity
Human MMP-2 cleaves the peptide substrate Mca-PLGL-Dpa-AR-NH2.

Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Neuroinflammation, Neuroscience, Stem Cells
Molecular Family    Enzymes and Regulators

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