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- 详细信息
- 文献和实验
- 技术资料
- 库存：
99
- 英文名：
Recombinant Human LIF (carrier-free)
- 供应商：
北京云肽生物科技有限公司
BioLegend 593904 Recombinant Human LIF (carrier-free)
北京云肽生物科技有限公司作为 BioLegend 的特约经销商，为广大客户提供原装正品，货期短，折扣好的服务准则。
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BioLegend 593904 Recombinant Human LIF (carrier-free)
产品简介：
产品品牌：BioLegend
产品货号：593904
产品名称：BioLegend 593904 Recombinant Human LIF (carrier-free)
产品规格：25 µg
产品说明 ：
Regulatory Status RUO
Other Names
Leukemia inhibitory factor, HILDA (human interleukin in DA cells), MLPLI (melanoma-derived LPL inhibitor), DIA (differentiation-inducing factor)
描述
白血病抑制因子（LIF）是一种20 kD的蛋白，属于IL-6受体家族。它与由LIF特异性亚基gp190或LIFR和与IL-6家族其他成员共享的亚基gp130组成的异二聚体膜受体结合。LIF复合物受体通过STAT3和JAK激酶发出信号。在包括胸腺、肺和神经元组织在内的各种组织中观察到LIF的表达。LIF表现出多种生物学效应，但最为人所知的是其抑制小鼠胚胎干细胞分化和促进干细胞自我更新的能力。人和小鼠LIF具有79%的序列同源性，并表现出跨物种活性。然而，LIF对干细胞分化的抑制似乎是小鼠特异性的。它参与诱导正常和髓系白血病细胞的造血分化、诱导神经元细胞分化、调节肾脏发育过程中的间充质向上皮转化，还可能在母婴界面的免疫耐受中发挥作用。对于该基因，已经观察到编码多种异构体的选择性剪接转录物变体。LIF可被促炎细胞因子如TNFα和IL-17上调，在类风湿性关节炎、神经损伤、全身炎症和结核病的病例中发现LIF水平升高。
Leukemia inhibitory factor (LIF) is a 20 kD protein that belongs to the IL-6 receptor family. It binds to a heterodimeric membrane receptor made up of a LIF-specific subunit, gp190 or LIFR, and the subunit gp130, which is shared with the other members of the IL-6 family. The LIF complex receptor signals though STAT3 and JAK kinases. LIF expression has been observed in various tissues including thymus, lung, and neuronal tissue. LIF displays diverse biological effects but is best known for its ability to inhibit the differentiation of embryonic stem cells in mice and contribute to stem cell self-renewal. Human and mouse LIF share 79% sequence homology and exhibit cross-species activity. However, LIF inhibition of stem cell differentiation appears to be mouse specific. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulation of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. LIF can be upregulated by pro-inflammatory cytokines such as TNFα and IL-17, and elevated levels of LIF have been found in cases of rheumatoid arthritis, neural injury, systemic inflammation, and tuberculosis.
Product Details
Source
Human LIF, amino acids (Ser23-Phe202) (Accession# NP_002300.1), was expressed in E. coli.
Molecular Mass
The 180 amino acid recombinant protein has a predicted molecular mass of approximately 20 kD. The protein migrates at about 20 kD in DTT-reducing conditions and about 18 kD in non-reducing condition by SDS-PAGE. The N-terminal amino acid is Ser.
Purity
>97%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS, pH 7.4.
Endotoxin Level
Less than 0.1 EU per µg of protein as determine by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
Human LIF induces proliferation of TF-1 cells. ED50 = 0.03-0.12 ng/ml, corresponding to a specific activity of 8.3-33.3 x 106 units/mg.
Application Bioassay
Application Notes
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
Distribution
Expressed in the trophectoderm of the developing embryo, activated CD4+ T cells.
Function
Inhibits embryonic stem cell differentiation, induces the terminal differentiation of myeloid leukemia cells. LIF is upregulated by TNFα and IL-17. LIF is downregulated by IL-4 and IL-13 in fibroblasts.
Interaction Embryonic stem cells.
Ligand/Receptor LIFR complex (gp190 and gp130).
Bioactivity
Human LIF induces proliferation of TF-1 cells.
Cell Type
Tregs, Hematopoietic stem and progenitors, Embryonic Stem Cells
Biology Area Cell Biology, Immunology, Stem Cells
Molecular Family Growth Factors, Cytokines/Chemokines
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BioLegend 593904 Recombinant Human LIF (carrier-free)
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